患有致死性A46性状的缺锌犊牛的淋巴细胞变化

Lymphocyte alterations in zinc-deficient calves with lethal trait A46.

作者信息

Perryman L E, Leach D R, Davis W C, Mickelsen W D, Heller S R, Ochs H D, Ellis J A, Brummerstedt E

机构信息

Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.

出版信息

Vet Immunol Immunopathol. 1989 Jul;21(3-4):239-48. doi: 10.1016/0165-2427(89)90034-2.

DOI:10.1016/0165-2427(89)90034-2

PMID:2800326

Abstract

Lymphocyte numbers and activities were evaluated at 2, 4, 8 and 12 weeks of age in two calves with lethal trait A46 (A46), a genetic disorder affecting intestinal zinc absorption. Plasma zinc concentrations declined to subnormal by 3 weeks of age, after which anorexia, diarrhea, alopecia and hyperkeratosis occurred. Lymphocyte response to phytohemagglutinin-P (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM) stimulation was variably reduced. CD4+ T-lymphocytes were subnormal on at least one observation period following onset of zinc deficiency, and relative numbers of B lymphocytes were decreased at 8 weeks. Secondary antibody responses to bacteriophage phi X 174 were significantly reduced. The results demonstrate that calves homozygous for the A46 trait have normal numbers of functional lymphocyte subpopulations at birth, and that the activity of their lymphocytes is altered once the calves become zinc deficient.

摘要

在两只患有致死性A46性状（A46）的犊牛中，于2周龄、4周龄、8周龄和12周龄时评估淋巴细胞数量和活性。A46是一种影响肠道锌吸收的遗传性疾病。血浆锌浓度在3周龄时降至正常水平以下，之后出现厌食、腹泻、脱毛和角化过度。淋巴细胞对植物血凝素-P（PHA）、刀豆蛋白A（Con A）和商陆有丝分裂原（PWM）刺激的反应有所降低。在缺锌开始后的至少一个观察期内，CD4 + T淋巴细胞数量低于正常水平，并且在8周龄时B淋巴细胞的相对数量减少。对噬菌体φX 174的二次抗体反应显著降低。结果表明，具有A46性状的纯合犊牛出生时功能性淋巴细胞亚群数量正常，并且一旦犊牛缺锌，其淋巴细胞活性就会发生改变。