Zhao Ran, So Masatomo, Maat Hendrik, Ray Nicholas J, Arisaka Fumio, Goto Yuji, Carver John A, Hall Damien
Research School of Chemistry, Australian National University, Acton ACT, 2601, Australia.
Institute for Protein Research, Osaka University, 3-1- Yamada-oka, Suita, Osaka, 565-0871, Japan.
Biophys Rev. 2016;8(4):445-471. doi: 10.1007/s12551-016-0233-7. Epub 2016 Nov 23.
Detection of amyloid growth is commonly carried out by measurement of solution turbidity, a low-cost assay procedure based on the intrinsic light scattering properties of the protein aggregate. Here, we review the biophysical chemistry associated with the turbidimetric assay methodology, exploring the reviewed literature using a series of pedagogical kinetic simulations. In turn, these simulations are used to interrogate the literature concerned with in vitro drug screening and the assessment of amyloid aggregation mechanisms.
淀粉样蛋白生长的检测通常通过测量溶液浊度来进行，这是一种基于蛋白质聚集体固有光散射特性的低成本检测方法。在此，我们回顾与比浊法检测方法相关的生物物理化学，通过一系列教学动力学模拟来探究已发表的文献。相应地，这些模拟被用于审视与体外药物筛选及淀粉样蛋白聚集机制评估相关的文献。
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