Hills L P, Tiffany T O
Clin Chem. 1980 Sep;26(10):1459-66.
We have developed a centrifugal analyzer with both fluorescence/light-scatter and conventional absorbance optics. The instrument is used in this investigation to study the formation of antigen-antibody complexes by light scattering and turbidimetric measurements, and to develop assays for human immunoglobulins G, A, and M. Concentrations are calculated from a nonlinear least-squares fit of calibrators, and antigen excess is automatically detected from kinetic curve characteristics. Precisions and patients' results are presented, and assay sensitivity and reliability in the detection of antigen excess are compared. We also investigated the effects of centrifugal force on complex formation. Both nephelometry and turbidimetry can be very satisfactorily adapted to centrifugal analyzers. We present a model to describe the observed differences between the light-scatter and the turbidity data.
我们开发了一种兼具荧光/光散射和传统吸光光学系统的离心分析仪。在本研究中，该仪器用于通过光散射和比浊法测量来研究抗原-抗体复合物的形成，并开发针对人免疫球蛋白G、A和M的检测方法。浓度通过校准物的非线性最小二乘法拟合计算得出，并且可根据动力学曲线特征自动检测抗原过量情况。文中给出了精密度和患者检测结果，并比较了检测抗原过量时的检测灵敏度和可靠性。我们还研究了离心力对复合物形成的影响。散射比浊法和比浊法都能非常令人满意地应用于离心分析仪。我们提出了一个模型来描述观察到的光散射数据和浊度数据之间的差异。
