Wang Yan, Wang Xiaoting, Shang Jin, Liu Huan, Yuan Yi, Guo Yu, Huang Bo, Zhou Yue
Department of Orthopedics, Xinqiao Hospital, Third Military Medical University, 183 Xinqiao Street, Shapingba District, 400037, Chongqing, China.
Department of Pharmacy, Urumqi General Hospital of Lanzhou Military Region, 359 North Youhao Road, Saybagh District, 830000, Urumqi, China.
Eur Spine J. 2017 Mar;26(3):884-893. doi: 10.1007/s00586-016-4898-1. Epub 2016 Dec 21.
To explore the effect of citric acid (CA)-1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)/N-hydroxysuccinimide (NHS) collagen gel on repairing annular defects.
Type I collagen was extracted from the rat-tail tendon and crosslinked with CA at different mass ratio using EDC and NHS as crosslinking reagents to prepare four kinds of collagen gels. Forty-eight adult SD rats were divided into first sham group (n = 8), second group (n = 10) which was punctured and injected with CA-EDC/NHS collagen gel, third group (n = 10) which was punctured and injected with CA-EDC/NHS collagen gel, fourth group (n = 10) which was punctured and injected with EDC/NHS collagen gel, and fifth group (n = 10) which was punctured and untreated. X-ray images and magnetic resonance imaging images were obtained before puncture and at the 1st, 2nd, and 4th week after puncture. At each time point, disc height index (%DHI), voxel count and modified MRI Pfirrmann grading were collected and analyzed. All animals were killed at the 4th week to study the morphology.
The discs in the second group showed only slight degeneration compared with the healthy discs, and the results of %DHI (average 79%), voxel count (average 126.9), Pfirrmann grading (average grade 1.3) and morphology in the second group indicated less degeneration tendency compared with the other three puncture groups at the 4th week (P < 0.05). The annular fibrosus was partially repaired by the collagen gels that bridged the defects.
CA-EDC/NHS collagen gel is capable of repairing annular defects induced by needle puncture, which may be closely related to the dose of CA.
探讨柠檬酸（CA）-1-乙基-3-（3-二甲氨基丙基）碳二亚胺（EDC）/N-羟基琥珀酰亚胺（NHS）胶原凝胶对修复环状缺损的作用。
从大鼠尾腱中提取I型胶原，以EDC和NHS作为交联剂，按不同质量比与CA交联制备4种胶原凝胶。将48只成年SD大鼠分为第一假手术组（n = 8）、第二组（n = 10），第二组进行穿刺并注射CA-EDC/NHS胶原凝胶、第三组（n = 10），第三组进行穿刺并注射CA-EDC/NHS胶原凝胶、第四组（n = 10），第四组进行穿刺并注射EDC/NHS胶原凝胶、第五组（n = 10），第五组进行穿刺但不做处理。在穿刺前以及穿刺后第1、2和4周获取X线图像和磁共振成像图像。在每个时间点，收集并分析椎间盘高度指数（%DHI）、体素计数和改良MRI Pfirrmann分级。所有动物在第4周处死以研究形态学。
与健康椎间盘相比，第二组椎间盘仅表现出轻微退变，第二组的%DHI（平均79%）、体素计数（平均126.9）、Pfirrmann分级（平均1.3级）和形态学结果表明，在第4周时与其他三个穿刺组相比退变倾向较小（P < 0.05）。胶原凝胶桥接缺损，部分修复了纤维环。
CA-EDC/NHS胶原凝胶能够修复针刺诱导的环状缺损，这可能与CA的剂量密切相关。
