Zhang Jun, Zhao Zhonghao, Hu Rong, Guo Lingfang, Zheng Limin, Du Zhenguo, Wu Zujian, Fang Shouguo, Zhang Songbai, Liu Yong
Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, 434025, Hubei, China.
Hubei Collaborative Innovation Center for Grain Industry, Yangtze University, Jingzhou, 434025, Hubei, China.
Arch Virol. 2017 Apr;162(4):1107-1111. doi: 10.1007/s00705-016-3196-x. Epub 2016 Dec 21.
A putative chrysovirus recovered from Brassica campestris var. purpurea and provisionally named "Brassica campestris chrysovirus 1" (BrcCV1) was sequenced. The genome of the putative BrcCV1 consists of three double-stranded RNAs (dsRNAs) comprising 3,639 (dsRNA 1), 3,567 (dsRNA 2) and 3,337 (dsRNA 3) base pairs, respectively, each containing a single open reading frame (ORF 1-3). The putative proteins encoded by ORF 1-3 show homologies to RdRp, CP and chryso-P3 of approved or tentative chrysoviruses. In addition, the three dsRNAs of BrcCV1 contain highly conserved 5' and 3' untranslated regions (UTRs) in a way similar to known chrysoviruses. In a phylogenetic tree based on the conserved amino acid sequences of the RdRps of chrysoviruses, totiviruses and partitiviruses, the putative BrcCV1 formed a separate clade with Raphanus sativus chrysovirus 1 (RasCV1), a putative trisegmented, plant-infecting chrysovirus, in the family Chrysoviridae.
从紫菜苔中分离出一种假定的金黄病毒，暂命名为“紫菜苔金黄病毒1”（BrcCV1），并对其进行了测序。假定的BrcCV1基因组由三条双链RNA（dsRNA）组成，分别包含3639个碱基对（dsRNA 1）、3567个碱基对（dsRNA 2）和3337个碱基对（dsRNA 3），每条链都含有一个单一的开放阅读框（ORF 1-3）。由ORF 1-3编码的假定蛋白质与已批准或暂定的金黄病毒的RdRp、CP和金黄病毒P3具有同源性。此外，BrcCV1的三条dsRNA含有高度保守的5'和3'非翻译区（UTR），其方式与已知的金黄病毒相似。在基于金黄病毒、全病毒和分体病毒RdRp保守氨基酸序列构建的系统发育树中，假定的BrcCV1与萝卜金黄病毒1（RasCV1）形成了一个单独的分支，RasCV1是一种假定的三分体、感染植物的金黄病毒，属于金黄病毒科。
