Xu Pengjun, Song Xueru, Yang Xianming, Tang Zhaoqi, Ren Guangwei, Lu Yanhui
Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, People's Republic of China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, People's Republic of China.
Arch Virol. 2017 Apr;162(4):1125-1128. doi: 10.1007/s00705-016-3195-y. Epub 2016 Dec 21.
The complete genome sequence of a novel single-stranded RNA virus in Nesidiocoris tenuis was determined by RNA-seq and rapid amplification of cDNA ends (RACE) methodologies and was named N. tenuis virus 1. The genomic RNA was 3970 nucleotides (nt) in length and contained two putative open reading frames (ORFs). ORF1 encoded a polypeptide with 283 amino acids containing a viral (superfamily 1) RNA helicase (Hel) domain, and ORF2 encoded a polypeptide with 294 amino acids containing an RNA-dependent RNA polymerase (RdRP) domain. Phylogenetic analysis using the deduced amino acid sequences indicated that the N. tenuis virus 1 clustered with Blackford virus; however, the low bootstrap values and unique genomic structure suggested that the virus is a prototype of a new type of unclassified viruses. The prevalence of N. tenuis virus 1 infection in field populations of N. tenuis differed between three locations, with 28.32% of the 113 sampled individuals testing positive for the virus.
通过RNA测序和cDNA末端快速扩增（RACE）方法，确定了西花蓟马中一种新型单链RNA病毒的完整基因组序列，并将其命名为西花蓟马病毒1。基因组RNA长度为3970个核苷酸（nt），包含两个推定的开放阅读框（ORF）。ORF1编码一个含有283个氨基酸的多肽，该多肽包含一个病毒（超家族1）RNA解旋酶（Hel）结构域，ORF2编码一个含有294个氨基酸的多肽，该多肽包含一个RNA依赖的RNA聚合酶（RdRP）结构域。使用推导的氨基酸序列进行的系统发育分析表明，西花蓟马病毒1与布莱克福德病毒聚类；然而，低自展值和独特的基因组结构表明该病毒是一种新型未分类病毒的原型。西花蓟马病毒1在西花蓟马田间种群中的感染率在三个地点有所不同，在113个采样个体中，有28.32%的个体对该病毒检测呈阳性。
