Díez Paula, Góngora Rafael, Orfao Alberto, Fuentes Manuel
a Department of Medicine and General Cytometry Service-Nucleus , Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL) , Salamanca , Spain.
b Proteomics Unit , Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL) , Salamanca , Spain.
Expert Rev Proteomics. 2017 Feb;14(2):137-146. doi: 10.1080/14789450.2017.1275967. Epub 2016 Dec 30.
B cell chronic lymphocytic leukemia (B-CLL) is a hematological malignancy considered as the most common leukemia in the Western world. The understanding of B cell differentiation is crucial for the diagnosis, prognosis, and treatment of the disease. Areas covered: In this review, B-cell ontogeny and its relation with the CLL development, in combination with the proteomic approaches which could provide a deep characterization of the disease through the characterization of the cellular signaling pathways involved in the pathological cells is described. Expert commentary: Although conventional strategies (genome sequencing, morphology assays, and immunophenotyping by flow cytometry and/or immunochemistry) have allowed the establishment of the disease stage based on different parameters, it is still necessary to utilize novel approaches (e.g., proteomics) that have the potential to simultaneously analyze thousands of molecules to improve understanding of CLL.
B细胞慢性淋巴细胞白血病（B-CLL）是一种血液系统恶性肿瘤，被认为是西方世界最常见的白血病。了解B细胞分化对于该疾病的诊断、预后和治疗至关重要。涵盖领域：在本综述中，描述了B细胞个体发生及其与CLL发展的关系，以及蛋白质组学方法，这些方法可通过对病理细胞中涉及的细胞信号通路进行表征，从而对该疾病进行深入表征。专家评论：尽管传统策略（基因组测序、形态学检测以及通过流式细胞术和/或免疫化学进行免疫表型分析）已能够根据不同参数确定疾病阶段，但仍有必要采用新方法（如蛋白质组学），这些方法有潜力同时分析数千种分子，以增进对CLL的了解。
