Division of Periodontology, College of Dentistry, The Ohio State University, Columbus, OH, USA.
J Clin Periodontol. 2017 Apr;44(4):394-402. doi: 10.1111/jcpe.12669. Epub 2017 Feb 11.
To investigate the gene expression profile of human gingiva following surgical wounding.
Ten volunteers had one side of the palate wounded. Five days later, biopsies were harvested from both wounded (healing gingiva) and contra-lateral site (normal gingiva). Tissue samples were processed for gene expression (RNA-Seq, real-time PCR) and immunohistochemistry. Gene set enrichment/pathway analysis was also performed.
Seven hundred genes were significantly differentially expressed in healing gingiva. Among genes with >twofold change (FC) in expression, 399 genes were up-regulated and 88 down-regulated, several not previously reported expressed in gingiva. Most increased in expression (≥30-FC) were MMP1, CCL18, SPP1, MUC21, CTHRC1, MMP10, and SERPINE1; most decreased (≥7-FC) were COCH, SIAH3, MT4, IGFL3, KY, and SYT16. Real-time PCR confirmed significantly changed mRNA levels for selective genes tested. Gene set enrichment analysis revealed several significantly enriched biological pathways. Immunohistochemistry confirmed protein expression of MUC21, CTHRC1, CTGF, and SYT16 in normal and healing gingiva.
This first comprehensive analysis of the human gingival transcriptome during surgical wound healing offers novel insights into the participating molecular and biological mechanisms. The present results could serve as basis for future investigations into gingival wound healing following surgical, traumatic, or other type of injury.
研究人牙龈在手术创伤后的基因表达谱。
10 名志愿者的一侧 palate 受伤。5 天后，从受伤侧（愈合牙龈）和对侧部位（正常牙龈）采集活检样本。对组织样本进行基因表达（RNA-Seq、实时 PCR）和免疫组织化学分析。还进行了基因集富集/通路分析。
700 个基因在愈合的牙龈中差异表达显著。在表达倍数变化（FC）大于 2 的基因中，399 个基因上调，88 个基因下调，其中一些以前未报道过在牙龈中表达。表达增加（≥30-FC）最多的是 MMP1、CCL18、SPP1、MUC21、CTHRC1、MMP10 和 SERPINE1；表达下调（≥7-FC）最多的是 COCH、SIAH3、MT4、IGFL3、KY 和 SYT16。实时 PCR 验证了所选基因测试的 mRNA 水平的显著变化。基因集富集分析显示了几个显著富集的生物学途径。免疫组织化学证实了 MUC21、CTHRC1、CTGF 和 SYT16 在正常和愈合牙龈中的蛋白表达。
这是第一次在人类牙龈创伤愈合过程中对转录组进行全面分析，为参与的分子和生物学机制提供了新的见解。本研究结果可为未来研究手术、创伤或其他类型损伤后牙龈伤口愈合提供基础。
