Zhang H, Li J L
Wei Sheng Wu Xue Bao. 1989 Apr;29(2):93-100.
A mycotoxin was extracted and purified from a strain of Fusarium moniliforme var. subglutinans isolated from mouldy corn seeds harvested in a serious Keshan disease region in Shaanxi Province. The purification procedure involves water extraction, ion exchange chromatography, desalination and crystallization. Its UV spectrum, IR spectrum and NMR are identical with that of the moniliformin. The toxin is highly toxic to young Beijing ducklings. The electrocardiogram (ECG) of the duckling was changed immediately after feeding with moniliformin. The cardiac muscle cells of Beijing ducklings were injured by the toxin. Toxicity may be alleviated to certain extent by applying adequate dosage of Selenium (3.18 X 10(-10) mol/L of H2SeO4) prior to moniliformin treatment. The permeability of the cardic muscle cells of the rats and young ducklings was damaged posterior to the injection of the toxin as tested by extracellular marcromolecular tracer (HRP) method. The relationship between moniliformin and Keshan disease was discussed. We consider that the moniliformin may be the causal agent of the disease.
从陕西省一个克山病重病区收获的霉变玉米种子中分离出的一株串珠镰刀菌禾谷变种中提取并纯化了一种霉菌毒素。纯化过程包括水提取、离子交换色谱、脱盐和结晶。其紫外光谱、红外光谱和核磁共振谱与串珠镰刀菌素的光谱相同。该毒素对北京雏鸭剧毒。用串珠镰刀菌素喂养雏鸭后，其心电图立即改变。北京雏鸭的心肌细胞受到该毒素损伤。在串珠镰刀菌素处理前给予适当剂量的硒（3.18×10⁻¹⁰ mol/L 的 H₂SeO₄），毒性可在一定程度上得到缓解。通过细胞外大分子示踪剂（HRP）法检测，注射毒素后大鼠和雏鸭心肌细胞的通透性受损。讨论了串珠镰刀菌素与克山病的关系。我们认为串珠镰刀菌素可能是该病的病原体。
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