Steyn M, Thiel P G, van Schalkwyk G C
J Assoc Off Anal Chem. 1978 May;61(3):578-80.
A bulk purification procedure is described for moniliformin, a mycotoxin produced by Fusarium moniliforme. The method involves methanol extraction of suitably molded maize, aqueous extraction of the methanol-free residue, ion exchange chromatography with a NaCl concentration gradient, desalination, and crystallization. A pKa value of 1.70 as well as molar absorptivities of 19100 (lambda (H2O) max 229 nm), 5600 (lambda (H2O) max 260 nm), and 4700 (lambda (MeOH) max 260 nm) L/mole/cm are reported for moniliformin.
本文描述了一种对串珠镰刀菌产生的霉菌毒素——串珠镰孢菌素进行大量纯化的方法。该方法包括用甲醇提取适当成型的玉米，对无甲醇残渣进行水提取，采用氯化钠浓度梯度进行离子交换色谱分离、脱盐和结晶。报告称串珠镰孢菌素的pKa值为1.70，摩尔吸光系数分别为19100（水相中λmax 229nm）、5600（水相中λmax 260nm）和4700（甲醇相中λmax 260nm）L/（mol·cm）。
