Singh Arun D, Aziz Hassan A, Pelayes David, Biscotti Charles V
*Cole Eye Institute, Cleveland, Ohio; †Department of Ophthalmology, Buenos Aires University and Maimonides University, Buenos Aires, ‡Department of Anatomic Pathology, Cleveland Clinic, Cleveland, Ohio.
Retina. 2017 Sep;37(9):1674-1677. doi: 10.1097/IAE.0000000000001425.
To report cytopathological observations on the cells retrieved from the 25-G cannula used during prognostic transvitreal fine-needle aspiration biopsy of choroidal melanoma.
Transvitreal fine-needle aspiration biopsy of choroidal melanoma was performed through a 25-G valved cannula. Twenty samples from 20 consecutive patients were obtained. Most tumors were treated with plaque radiation therapy (16/20, 80%) following standard clinical guidelines. Four enucleated globes (4/20, 20%) were subjected to a similar transvitreal biopsy before enucleation.
Cytopathological analysis of the cells retrieved from the cannula revealed the absence of any cells in 4 of 20 samples (20%). In the remaining 16 samples, definite melanoma cells and atypical cells (probable melanoma cells) were observed in 2 samples each (total 4, 25%). Histiocytes (4/16, 25%) and lymphocytes (1/16, 6%) were also observed. Thirteen samples (13/16, 81%) contained conjunctival epithelial epithelium. Prognostication could be performed on all fine-needle aspiration biopsy samples (20, 100%).
Use of a 25-G valved cannula offers potential advantages by isolating the needle tract and by allowing retrieval of the contaminating cells without affecting the prognostic yield of the fine-needle aspiration biopsy sample.
报告在脉络膜黑色素瘤预后性经玻璃体细针穿刺活检过程中，从25G套管中获取的细胞的细胞病理学观察结果。
通过25G带阀套管进行脉络膜黑色素瘤的经玻璃体细针穿刺活检。从20例连续患者中获取了20个样本。大多数肿瘤按照标准临床指南接受了敷贴放射治疗（16/20，80%）。4例眼球摘除标本（4/20，20%）在摘除前进行了类似的经玻璃体活检。
对从套管中获取的细胞进行细胞病理学分析，发现20个样本中有4个（20%）未发现任何细胞。在其余16个样本中，分别在2个样本中观察到明确的黑色素瘤细胞和非典型细胞（可能为黑色素瘤细胞）（共4个，25%）。还观察到组织细胞（4/16，25%）和淋巴细胞（1/16，6%）。13个样本（13/16，81%）含有结膜上皮。所有细针穿刺活检样本（20个，100%）均可进行预后评估。
使用25G带阀套管具有潜在优势，可隔离针道，并能获取污染细胞，而不影响细针穿刺活检样本的预后评估结果。
