Šlamberová R, Mikulecká A, Macúchová E, Hrebíčková I, Ševčíková M, Nohejlová K, Pometlová M
Department of Normal, Pathological and Clinical Physiology, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
Physiol Res. 2016 Dec 22;65(Suppl 5):S547-S555. doi: 10.33549/physiolres.933527.
The aim of the present study was to compare effect of three low doses of morphine (MOR) and delta9-tetrahydrocannabinol (THC) on social behavior tested in Social interaction test (SIT). 45 min prior to testing adult male rats received one of the drugs or solvents: MOR (1; 2.5; 5 mg/kg); saline as a solvent for MOR; THC (0.5; 1; 2 mg/kg); ethanol as a solvent for THC. Occurrence and time spent in specific patterns of social interactions (SI) and non-social activities (locomotion and rearing) was video-recorded for 5 min and then analyzed. MOR in doses of 1 and 2.5 mg/kg displayed decreased SI in total. Detailed analysis of specific patterns of SI revealed decrease in mutual sniffing and allo-grooming after all doses of MOR. The highest dose (5 mg/kg) of MOR decreased following and increased genital investigation. Rearing activity was increased by lower doses of MOR (1 and 2.5 mg/kg). THC, in each of the tested doses, did not induce any specific changes when compared to matching control group (ethanol). However, an additional statistical analysis showed differences between all THC groups and their ethanol control group when compared to saline controls. There was lower SI in total, lower mutual sniffing and allo-grooming, but higher rearing in THC and ethanol groups than in saline control group. Thus, changes seen in THC and ethanol groups are seemed to be attributed mainly to the effect of the ethanol. Based on the present results we can assume that opioids affect SI more than cannabinoid.
本研究的目的是比较三种低剂量吗啡（MOR）和δ9-四氢大麻酚（THC）对社交互动测试（SIT）中社交行为的影响。在测试前45分钟，成年雄性大鼠接受以下药物或溶剂之一：MOR（1；2.5；5毫克/千克）；生理盐水作为MOR的溶剂；THC（0.5；1；2毫克/千克）；乙醇作为THC的溶剂。对社交互动（SI）和非社交活动（运动和竖毛）的特定模式中出现的情况和所花费的时间进行5分钟的视频记录，然后进行分析。剂量为1和2.5毫克/千克的MOR总体上显示出SI减少。对SI的特定模式进行详细分析发现，所有剂量的MOR后相互嗅探和异体梳理均减少。MOR的最高剂量（5毫克/千克）减少了跟随行为并增加了生殖器检查。较低剂量的MOR（1和2.5毫克/千克）增加了竖毛活动。与匹配的对照组（乙醇）相比，THC在每个测试剂量下均未引起任何特定变化。然而，一项额外的统计分析表明，与生理盐水对照组相比，所有THC组与其乙醇对照组之间存在差异。THC组和乙醇组的总SI较低，相互嗅探和异体梳理较少，但竖毛行为比生理盐水对照组更高。因此，在THC组和乙醇组中看到的变化似乎主要归因于乙醇的作用。基于目前的结果，我们可以假设阿片类药物对SI的影响大于大麻素。
