Satellite DNAs are highly repetitive sequences that account for the majority of constitutive heterochromatin in many eukaryotic genomes. It is widely recognized that sequences and locations of satellite DNAs are highly divergent even in closely related species, contributing to the hypothesis that satellite DNA differences may underlie speciation. However, due to its repetitive nature, the mapping of satellite DNAs has been mostly left out of recent genomics analyses, hampering the use of molecular genetics techniques to better understand their role in speciation and evolution. Satellite DNAs are most extensively and comprehensively mapped in , a species that is also an excellent model system with which to study speciation. Yet the lack of comprehensive knowledge regarding satellite DNA identity and location in its sibling species (, , and ) has prevented the full utilization of in studying speciation. To overcome this problem, we initiated the mapping of satellite DNAs on the genomes of the species complex (, , , and ) using multi-color fluorescent hybridization (FISH) probes. Our study confirms a striking divergence of satellite DNAs in the species complex, even among the closely related species of the clade (, , and ), and suggests the presence of unidentified satellite sequences in these species.