Grupo de Fotônica e Fluidos Complexos, Instituto de Física, Universidade Federal de Alagoas, 57072-900, Maceió-AL, Brazil.
Fluorescence Imaging Group, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049, Madrid, Spain.
Adv Healthc Mater. 2017 Feb;6(4). doi: 10.1002/adhm.201601195. Epub 2016 Dec 23.
There is an urgent need to develop new diagnosis tools for real in vivo detection of first stages of ischemia for the early treatment of cardiovascular diseases and accidents. However, traditional approaches show low sensitivity and a limited penetration into tissues, so they are only applicable for the detection of surface lesions. Here, it is shown how the superior thermal sensing capabilities of near infrared-emitting quantum dots (NIR-QDs) can be efficiently used for in vivo detection of subcutaneous ischemic tissues. In particular, NIR-QDs make possible ischemia detection by high penetration transient thermometry studies in a murine ischemic hindlimb model. NIR-QDs nanothermometers are able to identify ischemic tissues by means of their faster thermal dynamics. In addition, they have shown to be capable of monitoring both the revascularization and damage recovery processes of ischemic tissues. This work demonstrates the applicability of fluorescence nanothermometry for ischemia detection and treatment, as well as a tool for early diagnosis of cardiovascular disease.
目前非常需要开发新的诊断工具，以便能够实时活体检测到缺血的早期阶段，从而对心血管疾病和事故进行早期治疗。然而，传统方法的灵敏度较低，对组织的穿透深度有限，因此仅适用于检测表面损伤。本研究展示了近红外发射量子点（NIR-QDs）卓越的热感应能力如何能够有效地用于活体检测皮下缺血组织。具体而言，NIR-QDs 使在小鼠缺血后肢模型中进行高穿透瞬态测温研究成为可能，从而实现了对缺血的检测。NIR-QDs 纳米温度计可以通过更快的热动力学来识别缺血组织。此外，它们还能够监测缺血组织的再血管化和损伤恢复过程。这项工作证明了荧光纳米测温在缺血检测和治疗中的适用性，以及在心血管疾病早期诊断中的应用。
