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[鸡胚心肌纤维收缩中局部变化的停顿的拍摄证据:细胞培养观察]

[Filmed evidence for locally changing pauses in the contraction of heart muscle fibrils in the chick embryo: a cell culture observation].

作者信息

Conti G, Gross W O

机构信息

Institut für allgemeine Histologie und Embryologie, Universität Freiburg, Schweiz.

出版信息

Acta Anat (Basel). 1989;135(4):361-4.

PMID:2801009
Abstract

In studies of the pulsation of embryonic heart muscle cells in culture, pauses can be sometimes observed during which, for some seconds, all interconnected cells rest. This can be remarked in the whole field of vision. At once the pulsation starts again and, with unbroken force, the activity continues until the next pause. Such pauses in the heart function of warm-blooded animals would be lethal. However, in culture, the rests can be limited to only one fibril of the cell complex. In that case, the performance of the heart would be maintained. Rests can be observed in even only a few sarcomeres, if not one single sarcomere of fibrils of the striated heart muscle. The sarcomeres are alternately exempted from contraction and the shortening of the fibril by the contraction is practically not diminished. This mechanism could provide a mode of recovery for the ever pulsating heart. The phenomenon of sarcomerical release from fibril contraction could be made visible in the film by a 4-fold slowdown.

摘要

在对培养的胚胎心肌细胞搏动的研究中,有时会观察到停顿现象,在此期间,所有相互连接的细胞会静止几秒钟。在整个视野中都能注意到这一点。随后搏动会立刻再次开始,并且以不间断的力量持续活动,直到下一次停顿。这种温血动物心脏功能中的停顿是致命的。然而,在培养环境中,静止状态可能仅限于细胞复合体的一条纤维。在这种情况下,心脏的功能会得以维持。即使只在少数肌节中能观察到静止状态,横纹肌心脏纤维甚至可能单个肌节都没有。肌节会交替地免于收缩,并且纤维收缩导致的缩短实际上并未减少。这种机制可以为不断搏动的心脏提供一种恢复模式。通过4倍慢放,肌节从纤维收缩中释放的现象在影片中可以清晰可见。

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