一种使用L-阿拉伯糖异构酶和D-来苏糖异构酶生产D-塔格糖和L-核糖的单步和两步异构化工艺。
A single and two step isomerization process for d-tagatose and l-ribose bioproduction using l-arabinose isomerase and d-lyxose isomerase.
作者信息
Patel Manisha J, Akhani Rekha C, Patel Arti T, Dedania Samir R, Patel Darshan H
机构信息
Department of Biochemistry, P. D. Patel Institute of Applied Sciences, CHARUSAT, Changa, 388 421, Gujarat, India.
Department of Biochemistry, P. D. Patel Institute of Applied Sciences, CHARUSAT, Changa, 388 421, Gujarat, India; Dr. K.C.Patel Research and Development Center, CHARUSAT, Changa, 388 421, Gujarat, India.
出版信息
Enzyme Microb Technol. 2017 Feb;97:27-33. doi: 10.1016/j.enzmictec.2016.11.001. Epub 2016 Nov 9.
l-ribose and d-tagatose are biochemically synthesized using sugar isomerases. The l-arabinose isomerase gene from Shigella flexneri (Sf-AI) was cloned and expressed in Escherichia coli BL-21. Sf-AI was applied for the bioproduction of d-tagatose from d-galactose. l-ribose synthesis was performed by two step isomerization using Sf-AI and d-lyxose/ribose isomerase from Cohnella laevoribosii. The overall 22.3% and 25% conversion rate were observed for d-tagatose and l-ribose production from d-galactose and l-arabinose respectively. In the present manuscript, synthesis of rare sugars from naturally available sugars is discussed along with the biochemical characterization of Sf-AI and its efficiency.
L-核糖和D-塔格糖通过糖异构酶进行生物合成。从弗氏志贺菌中克隆出L-阿拉伯糖异构酶基因（Sf-AI）并在大肠杆菌BL-21中表达。Sf-AI被用于从D-半乳糖生物生产D-塔格糖。L-核糖的合成通过两步异构化进行，使用来自左旋核糖科内拉菌的Sf-AI和D-来苏糖/核糖异构酶。从D-半乳糖和L-阿拉伯糖生产D-塔格糖和L-核糖的总转化率分别为22.3%和25%。在本手稿中，讨论了从天然可得糖合成稀有糖以及Sf-AI的生化特性及其效率。
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