Xu Hongtao, Liu Lili, Fan Xiaoting, Zhang Guanjun, Li Yuanchao, Jiang Biao
Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai 201210, PR China; State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, PR China.
Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai 201210, PR China.
Bioorg Med Chem Lett. 2017 Feb 1;27(3):505-510. doi: 10.1016/j.bmcl.2016.12.032. Epub 2016 Dec 11.
A diverse natural-product-like synthetic abietane diterpenoid library contains about 56 compounds were obtained, and evaluated for their potential in vitro cytotoxic or antitumor activity against A549, PC-3 and SKOV-3 tumor cell lines by SRB assay. Treatment of A549 cells with the most potent compound ketone 19 showed induction of apoptosis, as revealed by JC-1 mitochondrial membrane potential staining, TUNNEL assay, western blotting analysis and flow cytometry assay.
我们获得了一个包含约56种化合物的多样的类天然产物合成枞酸型二萜类化合物库，并通过SRB法评估了它们对A549、PC-3和SKOV-3肿瘤细胞系的体外细胞毒性或抗肿瘤活性。用最有效的化合物酮19处理A549细胞，通过JC-1线粒体膜电位染色、TUNNEL检测、蛋白质印迹分析和流式细胞术检测发现诱导了细胞凋亡。
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