Kinkel T, Lübbers H, Schmidt E, Molz P, Skrzipczyk H J
Hoechst AG, Frankfurt, FRG.
J Biolumin Chemilumin. 1989 Jul;4(1):136-9. doi: 10.1002/bio.1170040120.
Two new groups of chemiluminescent acridinium labels have been presented: (a) acridinium-9-thiocarboxylates, (b) acridinium-9-(N-sulphonyl)carboxamides. Both groups show higher light yields and faster emission kinetics compared with the known acridinium carboxylate. Owing to the poor storage stability of the thiocarboxylate tracers they are not suitable for commercial use. Acridinium-9-(N-sulphonyl)carboxamides allow a variation of the structure, their stability after conjugation to antibodies is in most cases clearly better than the stability of thiocarboxylate and carboxylate tracers. Light yields and emission kinetics are also superior and good immunoassays have been developed. Therefore, this new class of chemiluminescent labels seems to be suitable for use in commercial assays.
(a) 吖啶鎓-9-硫代羧酸盐，(b) 吖啶鎓-9-(N-磺酰基)羧酰胺。与已知的吖啶鎓羧酸盐相比，这两类标记物均显示出更高的发光产率和更快的发射动力学。由于硫代羧酸盐示踪剂的储存稳定性较差，它们不适合商业应用。吖啶鎓-9-(N-磺酰基)羧酰胺允许结构发生变化，在大多数情况下，它们与抗体偶联后的稳定性明显优于硫代羧酸盐和羧酸盐示踪剂的稳定性。其发光产率和发射动力学也更优，并且已经开发出了良好的免疫测定方法。因此，这类新的化学发光标记物似乎适用于商业检测。
