新型多取代芳基吖啶酯及其在免疫测定中的应用。

Novel poly-substituted aryl acridinium esters and their use in immunoassay.

作者信息

Law S J, Miller T, Piran U, Klukas C, Chang S, Unger J

机构信息

Ciba-Corning Diagnostics, E. Walpole, MA 02032.

出版信息

J Biolumin Chemilumin. 1989 Jul;4(1):88-98. doi: 10.1002/bio.1170040115.

DOI:10.1002/bio.1170040115

PMID:2801251

Abstract

A new series of stable acridinium ester conjugates have been developed for use as non-isotopic labels in immunoassay. They have proved to be a flexible alternative to radioimmunoassay. We present data showing the successful development of immunoassays in sandwich, competitive and receptor formats. In addition, hydrophilic acridinium ester analogues have been synthesized, encapsulated in liposomes, and utilized as labels in immunoassay. The potential of this technology is discussed.

摘要

已开发出一系列新型稳定的吖啶酯缀合物，用作免疫测定中的非同位素标记物。事实证明，它们是放射免疫测定的一种灵活替代方法。我们提供的数据表明，已成功开发出夹心、竞争和受体形式的免疫测定法。此外，已合成了亲水性吖啶酯类似物，将其包裹在脂质体中，并用作免疫测定中的标记物。本文讨论了该技术的潜力。