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Luciferase of Luciola mingrelica fireflies. Kinetics and regulation mechanism.

作者信息

Ugarova N N

机构信息

Chemistry Department, Lomonosov Moscow State University, USSR.

出版信息

J Biolumin Chemilumin. 1989 Jul;4(1):406-18. doi: 10.1002/bio.1170040155.

DOI:10.1002/bio.1170040155
PMID:2801227
Abstract

Biochemical properties, spectral parameters of bioluminescence and reaction kinetics for Luciola mingrelica firefly luciferase are described and analysed. The kinetic scheme of the enzymatic process is proposed and discussed. Allosteric regulation of luciferase activity by ATP and its analogues is considered and binding Mg2+ to luciferase shown to increase its activity. Regulation mechanism of luciferase activity by phospholipids is analysed and choline-containing phospholipids shown to be specific luciferase activators. Some properties of firefly luciferase and the luciferase synthesized during firefly mRNA translation in frog oocytes are compared.

摘要

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