Ameeramja Jaishabanu, Perumal Ekambaram
Molecular Toxicology Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore, 641 046, India.
Molecular Toxicology Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore, 641 046, India.
Food Chem Toxicol. 2017 Nov;109(Pt 2):941-950. doi: 10.1016/j.fct.2016.12.024. Epub 2016 Dec 22.
The present study is aimed to determine the potential benefits of protocatechuic acid methyl ester (PCAME) against fluoride (F) induced lung toxicity using A549 cells. The cells were treated with sodium fluoride (NaF) alone and in combination with PCAME for different time points (0-24 h) and evaluated for intracellular reactive oxygen species (ROS) production, F content, oxidative stress markers, apoptosis and mRNA expression of redox signaling and inflammatory genes. The results shown that PCAME alleviates the toxic effects of Fvia modulating its bioavailability, intracellular calcium level, mitochondrial membrane integrity and redox signaling in A549 cells. F induced changes in ROS generation, oxidative stress markers, TUNEL positive cells and mRNA levels of inflammatory genes were further normalized by PCAME. Taken together, these findings revealed that PCAME effectively attenuated the F induced changes in oxidative stress, inflammation and apoptosis markers by reducing its cellular content, ROS generation and biochemical and molecular changes. Thus PCAME can be used as a nutraceutical agent for F toxicity.
本研究旨在利用A549细胞确定原儿茶酸甲酯（PCAME）对抗氟（F）诱导的肺毒性的潜在益处。细胞分别单独用氟化钠（NaF）处理以及与PCAME联合处理不同时间点（0 - 24小时），并评估细胞内活性氧（ROS）生成、氟含量、氧化应激标志物、细胞凋亡以及氧化还原信号和炎症基因的mRNA表达。结果表明，PCAME通过调节其生物利用度、细胞内钙水平、线粒体膜完整性和A549细胞中的氧化还原信号，减轻了氟的毒性作用。PCAME进一步使氟诱导的ROS生成、氧化应激标志物、TUNEL阳性细胞和炎症基因mRNA水平的变化恢复正常。综上所述，这些发现表明，PCAME通过降低其细胞含量、ROS生成以及生化和分子变化，有效减轻了氟诱导的氧化应激、炎症和细胞凋亡标志物的变化。因此，PCAME可作为一种抗氟毒性的营养剂。
