Molecular Toxicology Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore, 640 046, India.
Inflammation. 2018 Jun;41(3):886-895. doi: 10.1007/s10753-018-0743-5.
Fluorosis (fluoride toxicity) seems to be reduced by plant secondary metabolites. Tamarind seed coat extract (TSCE), a mixture of procyanidins and polyphenols, possesses numerous pharmacological activities. Regardless of its beneficial properties, the underlying molecular mechanism against chronic fluorosis in vivo is not known. Hence, the present study is aimed to investigate the efficacy of TSCE against fluoride (F)-induced pulmonary toxicity in rats. Female Wistar rats were randomly divided into four groups and treated with NaF (300 ppm in drinking water) and TSCE (100 mg/kg/bw by oral intubation) alone and in combination daily for 30 days. The results showed that F exposure-induced modifications in lung injury markers in both serum and BALF were restored by TSCE supplementation. Additionally, F-induced changes in oxidative stress (NOX4 and p38α MAPK), inflammation (NF-κB, COX-2, and HO-1), apoptosis (Hsp27, Hsp60, caspase3p20, and PARP1), and fibrosis (TGF-β1, psmad3, Col1αl, and hydroxyproline level) markers in the lungs were modulated by TSCE. Thus, TSCE offers protection against F-induced pulmonary inflammation and fibrosis in rats.
氟中毒（氟化物毒性）似乎可以通过植物次生代谢产物减轻。罗望子种皮提取物（TSCE）是原花青素和多酚的混合物，具有多种药理活性。尽管它具有有益的特性，但体内针对慢性氟中毒的潜在分子机制尚不清楚。因此，本研究旨在研究 TSCE 对大鼠氟（F）诱导的肺毒性的疗效。雌性 Wistar 大鼠随机分为四组，单独和联合用 NaF（饮用水中的 300 ppm）和 TSCE（口服灌胃 100 mg/kg/bw）处理 30 天。结果表明，TSCE 补充可恢复 F 暴露诱导的血清和 BALF 中肺损伤标志物的改变。此外，TSCE 调节了 F 诱导的肺中氧化应激（NOX4 和 p38α MAPK）、炎症（NF-κB、COX-2 和 HO-1）、凋亡（Hsp27、Hsp60、caspase3p20 和 PARP1）和纤维化（TGF-β1、psmad3、Col1αl 和羟脯氨酸水平）标志物的变化。因此，TSCE 为预防大鼠 F 诱导的肺炎症和纤维化提供了保护。
