对小鼠巨噬细胞上与膜相关的白细胞介素-1样活性的研究。

Investigations into the membrane associated IL-1 like activity on mouse macrophages.

作者信息

Kidgell A E, Paska W, Humphray H P, Mackenzie A R

机构信息

Department of Cellular Sciences, Glaxo Group Research Ltd. Greenford Middlesex, UK.

出版信息

Agents Actions. 1989 Jun;27(3-4):285-6. doi: 10.1007/BF01972799.

DOI:10.1007/BF01972799

PMID:2801312

Abstract

The present study was undertaken to investigate the expression of membrane IL-1 (mIL-1) in mouse macrophages. Membrane IL-1 activity was measured on paraformaldehyde fixed peritoneal macrophages using EL4 6.1 lymphocyte cell line. Acid washing prior to fixation did not remove the mIL-1 activity, indicating that mIL-1 is an integral part of the membrane. Adherence to tissue culture plastic plates was found to be a potent inducer of mIL-1 activity in both resident and C. parvum activated macrophages. Dexamethasone reduced mIL-1 activity induced in vivo by C. parvum and also that induced by adherence in vitro.

摘要

本研究旨在调查膜白细胞介素-1（mIL-1）在小鼠巨噬细胞中的表达。使用EL4 6.1淋巴细胞系在经多聚甲醛固定的腹膜巨噬细胞上测量膜白细胞介素-1活性。固定前的酸洗并未去除mIL-1活性，表明mIL-1是膜的一个组成部分。发现贴附于组织培养塑料板是驻留型和微小隐孢子虫激活的巨噬细胞中mIL-1活性的有效诱导剂。地塞米松降低了微小隐孢子虫在体内诱导的mIL-1活性以及体外贴附诱导的mIL-1活性。