Yektaş Abdulkadir, Çabalar Murat, Sar Mehmet, Alagöl Ayşin, Çelik Duygu Sultan, Yayla Vildan, Tolga Deniz
Bagcilar Training and Research Hospital, Istanbul, Turkey.
Bakırköy Dr. Sadikonuk eğitim ve araştırma hastanesi, İstanbul, Turkey.
Braz J Anesthesiol. 2017 Jan-Feb;67(1):57-66. doi: 10.1016/j.bjane.2015.08.012. Epub 2016 Apr 28.
The present study was designed to test the hypothesis that high dose dexmedetomidine would increase the duration of antinociception to a thermal stimulus in a rat model of sciatic nerve blockade without causing nerve damage. The rats were anesthetized with isoflurane. After electromyography (EMG) recordings, right sciatic nerves were explored and perineural injections were delivered: Group D (n=7), 40μgμgkg dexmedetomidine administration, Group II (n=6), (0.2mL) saline administration, Group III (n=2), only surgically exploration of the right sciatic nevre. Time to paw withdrawal latency (PAW) to a thermal stimulus for both paws and an assessment of motor function were measured every 30min after the nerve block until a return to baseline. The compound muscle action potential (CMAP) of right and left sciatic nerves were recorded 10 times per each nerve once more after perineural injections at 14 day. After EMG recordings, right and the part of left sciatic nerve were excised at a length of at minimum 15mm for histopathological examination. Comparison of right/left CMAP amplitude ratios before and 14 days after the procedure showed a statistically significant difference (p=0.000). There were no differences in perineural inflammation between the Group D, Group S, and Group E at 14 days.
在大鼠坐骨神经阻滞模型中，高剂量右美托咪定可延长对热刺激的镇痛持续时间，且不会造成神经损伤。大鼠用异氟烷麻醉。在进行肌电图（EMG）记录后，暴露右侧坐骨神经并进行神经周围注射：D组（n = 7），给予40μg/kg右美托咪定；II组（n = 6），给予（0.2mL）生理盐水；III组（n = 2），仅对右侧坐骨神经进行手术探查。神经阻滞后，每隔30分钟测量双爪对热刺激的爪缩反应潜伏期（PAW）并评估运动功能，直至恢复到基线水平。在神经周围注射14天后，再次对左右坐骨神经各记录10次复合肌肉动作电位（CMAP）。在进行EMG记录后，切除右侧及左侧部分坐骨神经，长度至少为15mm，用于组织病理学检查。比较手术前后右/左CMAP振幅比值，差异具有统计学意义（p = 0.000）。在第14天，D组、S组和E组之间神经周围炎症无差异。
