Brigham K L, Meyrick B, Christman B, Magnuson M, King G, Berry L C
Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee.
Am J Med Sci. 1989 Oct;298(4):278-81. doi: 10.1097/00000441-198910000-00013.
The authors report successful in vivo transfection of lungs of mice with a functioning prokaryotic gene encoding the intracellular enzyme, chloramphenicol acetyltransferase (CAT). Transfection was accomplished by injecting a plasmid containing the coding region for CAT driven by the SV40 early promoter (pSV2CAT) complexed to specially synthesized cationic liposomes. Intravenous or intratracheal injection of DNA-liposomes resulted in expression of the CAT gene in the lungs, persisting for at least a week, with little enzyme activity detectable in systemic organs. This method should permit either transient or stable in vivo transfection of the lungs with a gene encoding any protein of interest, providing a powerful experimental tool and potentially a novel and broadly applicable clinical therapeutic technique.
作者报告了用编码细胞内酶氯霉素乙酰转移酶（CAT）的有功能原核基因成功地在小鼠肺中进行体内转染。转染是通过注射一种质粒来完成的，该质粒含有由SV40早期启动子驱动的CAT编码区（pSV2CAT），它与特别合成的阳离子脂质体复合。静脉内或气管内注射DNA-脂质体导致肺中CAT基因的表达，持续至少一周，在全身器官中几乎检测不到酶活性。该方法应能实现用编码任何感兴趣蛋白质的基因对肺进行体内瞬时或稳定转染，提供一种强大的实验工具，并有可能成为一种新颖且广泛适用的临床治疗技术。
