Wei Xiaoming, Kong Cihang, Sy Samuel, Ko Ho, Tsia Kevin K, Wong Kenneth K Y
Department of Electrical and Electronic Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, China.
Department of Electronic Engineering, Faculty of Engineering, The Chinese University of Hong Kong, Hong Kong, China.
Biomed Opt Express. 2016 Nov 18;7(12):5208-5217. doi: 10.1364/BOE.7.005208. eCollection 2016 Dec 1.
Optical glass fiber has played a key role in the development of modern optical communication and attracted the biotechnology researcher's great attention because of its properties, such as the wide bandwidth, low attenuation and superior flexibility. For ultrafast optical imaging, particularly, it has been utilized to perform MHz time-stretch imaging with diffraction-limited resolutions, which is also known as serial time-encoded amplified microscopy (STEAM). Unfortunately, time-stretch imaging with dispersive fibers has so far mostly been demonstrated at the optical communication window of 1.5 μm due to lack of efficient dispersive optical fibers operating at the shorter wavelengths, particularly at the bio-favorable window, i.e., <1.0 μm. Through fiber-optic engineering, here we demonstrate a 7.6-MHz dual-color time-stretch optical imaging at bio-favorable wavelengths of 932 nm and 466 nm. The sensitivity at such a high speed is experimentally identified in a slow data-streaming manner. To the best of our knowledge, this is the first time that all-optical time-stretch imaging at ultrahigh speed, high sensitivity and high chirping rate (>1 ns/nm) has been demonstrated at a bio-favorable wavelength window through fiber-optic engineering.
光学玻璃纤维在现代光通信的发展中发挥了关键作用,并因其诸如宽带宽、低衰减和卓越柔韧性等特性而引起了生物技术研究人员的极大关注。特别是对于超快光学成像,它已被用于执行具有衍射极限分辨率的兆赫兹时间拉伸成像,这也被称为串行时间编码放大显微镜(STEAM)。不幸的是,由于缺乏在较短波长(特别是在生物适宜窗口,即<1.0μm)工作的高效色散光纤,到目前为止,使用色散光纤的时间拉伸成像大多在1.5μm的光通信窗口进行演示。通过光纤工程,我们在此展示了在932nm和466nm的生物适宜波长下的7.6兆赫兹双色时间拉伸光学成像。以慢数据流的方式通过实验确定了如此高速下的灵敏度。据我们所知,这是首次通过光纤工程在生物适宜波长窗口展示超高速、高灵敏度和高啁啾率(>1ns/nm)的全光时间拉伸成像。