Blanco L N, Massaro G D, Massaro D
Calvin and Flavia Oak Asthma Research and Treatment Facility, Pulmonary Research Laboratories, University of Miami School of Medicine 33136.
Am J Physiol. 1989 Oct;257(4 Pt 1):L240-7. doi: 10.1152/ajplung.1989.257.4.L240.
We used three-dimensional reconstruction 1) to determine the effect of treating rats with dexamethasone, from age 4 to 13 days, on alveolar volume (v) and number (Na) and 2) to determine if v, Na, or both change between age 14 and 60 days. At age 14 days, v, Na, and gas exchange surface area (Sa) were (2.7 +/- 0.3) x 10(4) microns3, (20.2 +/- 2.1) x 10(6), and 832 +/- 29 cm2, respectively, in diluent-treated rats; in dexamethasone-treated 14-day-old rats the same parameters were (7.5 +/- 1.1) x 10(4) microns3, (9.9 +/- 1.6) x 10(6), and 733 +/- 16 cm2, respectively. At age 60 days, v, Na, and Sa were (7.1 +/- 0.4) x 10(4) microns3, (60.8 +/- 4.1) x 10(6), and 4,495 +/- 187 cm2 in diluent-treated rats and in rats treated with dexamethasone from age 4 to 13 days, v, Na, and Sa at age 60 days were (15.9 +/- 2.4) x 10(4) microns3, (25.8 +/- 3.8) x 10(6), and 3,424 +/- 203 cm2. We conclude that treatment with dexamethasone from age 4 to 13 days (the period of normal septation) resulted in larger alveoli at age 14 and 60 days, and in diluent- and dexamethasone-treated rats the increase in Sa between age 14 and 60 days is due, at least in part, to the formation of new alveoli.
1）确定4至13日龄大鼠接受地塞米松治疗对肺泡体积（v）和数量（Na）的影响；2）确定v、Na或两者在14至60日龄之间是否发生变化。在14日龄时，用稀释剂处理的大鼠的v、Na和气体交换表面积（Sa）分别为（2.7±0.3）×10⁴立方微米、（20.2±2.1）×10⁶、和832±29平方厘米；在14日龄接受地塞米松治疗的大鼠中，相同参数分别为（7.5±1.1）×10⁴立方微米、（9.9±1.6）×10⁶、和733±16平方厘米。在60日龄时，用稀释剂处理的大鼠的v、Na和Sa分别为（7.1±0.4）×10⁴立方微米、（60.8±4.1）×10⁶、和4495±187平方厘米，而在4至13日龄接受地塞米松治疗的大鼠中，60日龄时的v、Na和Sa分别为（15.9±2.4）×10⁴立方微米、（25.8±3.8）×10⁶、和3424±203平方厘米。我们得出结论，4至13日龄（正常分隔期）接受地塞米松治疗，导致14和60日龄时肺泡更大，并且在稀释剂处理组和地塞米松处理组大鼠中，14至60日龄之间Sa的增加至少部分归因于新肺泡的形成。
