Wanebo H J, Jones T, Pace R, Cantrell R, Levine P
Roger Williams General Hospital, Brown University, Providence, Rhode Island 02908.
Am J Surg. 1989 Oct;158(4):356-60. doi: 10.1016/0002-9610(89)90133-5.
Patients with head and neck squamous cell carcinoma commonly have depressed cell-mediated immunity which is known to correlate with ultimate prognosis. Selective immune studies were conducted in 27 head and neck cancer patients to determine the potential of interleukin-2 as an immune restorative agent. Patients showed the expected depression of lymphocyte proliferation to phytohemagglutinin and had borderline depressed natural killer cell activity and relatively normal interleukin-2 production. Addition of interleukin-2 at 100 units/ml markedly enhanced natural killer cell activity to normal levels. Serum from head and neck patients was also immune-suppressive. Heat-inactivated serum depressed lymphocyte proliferation and natural killer cell activity of control leukocytes. Lymphocyte incubation with interleukin-2 significantly counteracted immune suppressive serum effects and restored depressed lymphocyte function to normal levels. The effective in vitro interleukin-2 dose is potentially achievable by infusion at approximate doses of 3 X 10(6) units/M2.
头颈部鳞状细胞癌患者通常存在细胞介导免疫功能低下的情况，已知这与最终预后相关。对27名头颈部癌症患者进行了选择性免疫研究，以确定白细胞介素-2作为免疫恢复剂的潜力。患者对植物血凝素的淋巴细胞增殖表现出预期的抑制，自然杀伤细胞活性处于临界抑制状态，白细胞介素-2产生相对正常。添加100单位/毫升的白细胞介素-2可显著将自然杀伤细胞活性提高到正常水平。头颈部患者的血清也具有免疫抑制作用。热灭活血清可抑制对照白细胞的淋巴细胞增殖和自然杀伤细胞活性。淋巴细胞与白细胞介素-2孵育可显著抵消免疫抑制血清的作用，并将受抑制的淋巴细胞功能恢复到正常水平。通过以约3×10(6)单位/平方米的剂量输注，有可能达到体外有效的白细胞介素-2剂量。
