Department of Microbiology, Faculty of Medicine, Government Theni Medical College, Tamil Nadu Dr M.G.R. Medical University, Theni 625512, India; Department of Microbiology, Faculty of Medicine, Dr A.L.M. Post Graduate Institute of Basic Medical Sciences, University of Madras, Chennai 600113, India.
Department of Microbiology, Faculty of Medicine, Dr A.L.M. Post Graduate Institute of Basic Medical Sciences, University of Madras, Chennai 600113, India.
J Glob Antimicrob Resist. 2017 Mar;8:97-101. doi: 10.1016/j.jgar.2016.10.010. Epub 2016 Dec 23.
The aim of this study was to validate the use of LML antimicrobial gradient strips for quantitative determination of carbapenem susceptibility in Enterobacteriaceae.
A total of 95 non-redundant Enterobacteriaceae strains isolated during 2012-2014 were used for this validation study. Initially, LML antimicrobial gradient strips were validated for their performance in comparison with the agar dilution method. The test strip was then validated in comparison with broth microdilution (BMD) and Etest with 24 selected strains using the same inocula and other laboratory parameters.
The LML strip showed 83%, 68% and 86% essential agreement (within ±1 log dilution) with the reference methods of agar dilution, BMD and Etest, respectively; furthermore, essential agreement was >90% within ±2 log dilution. Categorical agreement was ≥87% with all reference methods according to Clinical and Laboratory Standards Institute (CLSI) and European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints. However, the meropenem strip requires performance improvements to fulfil US Food and Drug Administration (FDA) and International Organization for Standardization (ISO) requirements for an antimicrobial susceptibility test device.
In LML antimicrobial gradient strip minimum inhibitory concentrations (MICs) were comparable with Etest MICs and it might serve as a reasonable, cost-effective alternative to Etest for quantitative determination of carbapenem susceptibility in Enterobacteriaceae.
本研究旨在验证 LML 抗菌梯度条在肠杆菌科碳青霉烯类药物药敏定量测定中的应用。
本验证研究共使用了 2012 年至 2014 年期间分离的 95 株非冗余肠杆菌科菌株。首先，与琼脂稀释法比较，对 LML 抗菌梯度条的性能进行了验证。然后，使用相同的接种物和其他实验室参数，用 24 株选定的菌株对测试条进行了肉汤微量稀释（BMD）和 Etest 的验证。
LML 条与琼脂稀释、BMD 和 Etest 参考方法的符合率分别为 83%、68%和 86%（±1 对数稀释范围内）；此外，在±2 对数稀释范围内符合率>90%。根据临床和实验室标准协会（CLSI）和欧洲抗菌药物敏感性试验委员会（EUCAST）的折点，与所有参考方法的分类符合率均≥87%。然而，美罗培南条需要改进性能，以满足美国食品和药物管理局（FDA）和国际标准化组织（ISO）对抗菌药敏试验设备的要求。
在 LML 抗菌梯度条中，最低抑菌浓度（MIC）与 Etest MIC 相当，它可能是一种合理且具有成本效益的替代 Etest 的方法，可用于定量测定肠杆菌科的碳青霉烯类药物敏感性。
