Yoshida M, Osame M, Kawai H, Toita M, Kuwasaki N, Nishida Y, Hiraki Y, Takahashi K, Nomura K, Sonoda S
Department of Viral Oncology, Cancer Institute, Tokyo, Japan.
Ann Neurol. 1989 Sep;26(3):331-5. doi: 10.1002/ana.410260304.
To estimate the replication of the human T-cell leukemia virus type I (HTLV-I) in patients with HTLV-I-associated myelopathy (HAM), or tropical spastic paraparesis (TSP), HTLV-I DNA integrated into lymphocyte genomes was analyzed by Southern blot hybridization. HTLV-I DNA was detected in 125 (82%) of 153 patients and most showed random integration. This incidence was much higher than the 29% found in asymptomatic carriers. Therefore, HAM/TSP development is associated with a high level of HTLV-I replication. In addition, lymphocytes from 3 patients with HAM/TSP showed monoclonal integration of HTLV-I DNA, indicating adult T-cell leukemia.
为评估人类I型T细胞白血病病毒（HTLV-I）在HTLV-I相关脊髓病（HAM）或热带痉挛性截瘫（TSP）患者中的复制情况，通过Southern印迹杂交分析整合到淋巴细胞基因组中的HTLV-I DNA。在153例患者中的125例（82%）检测到HTLV-I DNA，且大多数表现为随机整合。这一发生率远高于无症状携带者中发现的29%。因此，HAM/TSP的发生与高水平的HTLV-I复制相关。此外，3例HAM/TSP患者的淋巴细胞显示HTLV-I DNA的单克隆整合，提示成人T细胞白血病。
