Mascitti Marco, Santarelli Andrea, Zizzi Antonio, Procaccini Maurizio, Lo Muzio Lorenzo, Rubini Corrado
Department of Clinical Specialistic and Dental Sciences, Marche Polytechnic University.
J Oral Sci. 2016;58(4):459-464. doi: 10.2334/josnusd.16-0147.
Odontogenic tumors are a group of lesions arising from the odontogenic apparatus. Although the mechanism of oncogenesis and tumor progression in these lesions remains unknown, certain proteins, such as those involved in apoptosis, seem to be involved in the differentiation and proliferation of odontogenic epithelial cells. The aim of this study was to analyze the expression of p73 and TNF-related apoptosis-inducing ligand (TRAIL) in odontogenic tumors and cysts, and to clarify changes in the expression of these proteins. Immunohistochemical analysis was performed on 21 ameloblastomas, 15 keratocystic odontogenic tumors and 15 dentigerous cysts. We carried out quantitative assessment of p73 and TRAIL expression by determining the percentages of positive cells on a continuous scale. Five cases of orthokeratinized odontogenic cyst were also examined. The percentages of cells immunohistochemically positive for p73 were 52.6 ± 25.4% in ameloblastomas, 76.0 ± 13.1% in keratocystic odontogenic tumors, and 26.7 ± 30.7% in odontogenic cysts, whereas the corresponding figures for TRAIL were 57.6 ± 16.1%, 8.9 ± 10.0%, and 1.5 ± 0.5%, respectively. Imbalance of the apoptosis pathway, with dysregulation of p73 and TRAIL, seems to play a role in the oncogenesis of odontogenic tumors.(J Oral Sci 58, 459-464, 2016).
牙源性肿瘤是一组起源于牙源性器官的病变。尽管这些病变的肿瘤发生和进展机制尚不清楚，但某些蛋白质，如参与细胞凋亡的蛋白质，似乎参与了牙源性上皮细胞的分化和增殖。本研究的目的是分析p73和肿瘤坏死因子相关凋亡诱导配体（TRAIL）在牙源性肿瘤和囊肿中的表达，并阐明这些蛋白质表达的变化。对21例成釉细胞瘤、15例牙源性角化囊性瘤和15例含牙囊肿进行了免疫组织化学分析。我们通过连续测定阳性细胞百分比对p73和TRAIL表达进行了定量评估。还检查了5例正角化牙源性囊肿。成釉细胞瘤中p73免疫组化阳性细胞百分比为52.6±25.4%，牙源性角化囊性瘤中为76.0±13.1%，牙源性囊肿中为26.7±30.7%，而TRAIL的相应数字分别为57.6±16.1%、8.9±10.0%和1.5±0.5%。凋亡途径的失衡，伴随着p73和TRAIL的失调，似乎在牙源性肿瘤的发生中起作用。（《口腔科学杂志》58卷，459 - 464页，2016年）
