单次低剂量电离辐射可诱导成年斑马鱼及其未受辐射的后代产生遗传毒性。
Single Low-Dose Ionizing Radiation Induces Genotoxicity in Adult Zebrafish and its Non-Irradiated Progeny.
作者信息
Lemos J, Neuparth T, Trigo M, Costa P, Vieira D, Cunha L, Ponte F, Costa P S, Metello L F, Carvalho A P
机构信息
ICBAS - Institute of Biomedical Sciences, University of Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313, Porto, Portugal.
Nuclear Medicine Department, High Institute for Allied Health Technologies of Porto - Polytechnic Institute of Porto (ESTSP.IPP), Rua Valente Perfeito 322, 4400-330, Vila Nova de Gaia, Portugal.
出版信息
Bull Environ Contam Toxicol. 2017 Feb;98(2):190-195. doi: 10.1007/s00128-016-2006-1. Epub 2016 Dec 26.
This study investigated to what extent a single exposure to low doses of ionizing radiation can induce genotoxic damage in irradiated adult zebrafish (Danio rerio) and its non-irradiated F1 progeny. Four groups of adult zebrafish were irradiated with a single dose of X-rays at 0 (control), 100, 500 and 1000 mGy, respectively, and couples of each group were allowed to reproduce following irradiation. Blood of parental fish and whole-body offspring were analysed by the comet assay for detection of DNA damage. The level of DNA damage in irradiated parental fish increased in a radiation dose-dependent manner at day 1 post-irradiation, but returned to the control level thereafter. The level of DNA damage in the progeny was directly correlated with the parental irradiation dose. Results highlight the genotoxic risk of a single exposure to low-dose ionizing radiation in irradiated individuals and also in its non-irradiated progeny.
本研究调查了单次低剂量电离辐射在多大程度上可诱导受辐照成年斑马鱼（Danio rerio）及其未受辐照的F1代子代产生遗传毒性损伤。四组成年斑马鱼分别接受0（对照）、100、500和1000毫戈瑞的单次X射线照射，每组照射后配对繁殖。通过彗星试验分析亲代鱼的血液和子代的全身，以检测DNA损伤。受辐照亲代鱼的DNA损伤水平在辐照后第1天呈辐射剂量依赖性增加，但此后恢复到对照水平。子代的DNA损伤水平与亲代辐照剂量直接相关。结果突出了单次低剂量电离辐射对受辐照个体及其未受辐照子代的遗传毒性风险。
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