Musthafa M Saiyad, Athaullah A, Anbumani S, Ali A Jawahar, War Mehrajuddin, Paray Bilal Ahmad, Al-Sadoon Mohammad K, Muthiah S S, Kembeeram Palani, Harikrishnan R
P. G. & Research Department of Zoology, The New College, Chennai 600 014, Tamilnadu, India.
P. G. & Research Department of Zoology, The New College, Chennai 600 014, Tamilnadu, India.
Appl Radiat Isot. 2017 Oct;128:108-113. doi: 10.1016/j.apradiso.2017.07.002. Epub 2017 Jul 5.
Using Zebrafish (Danio rerio) as a model organism, we evaluated the radioprotective and antioxidant effects of the Indian traditional medicine Shilajit exposed to X-Ray. The Zebrafish were divided into three experimental groups and control group, each group containing ten fish. The three experimental fish groups, group I, group II and group III were fed with 3, 5 and 7ppm shilajit encapsulated Chironomous larvae and group IV served as a control fed with non- encapsulated larvae. After 60 days of feeding trial, fish were irradiated with X-Ray at a single acute dose of 1Gy. 72h of post-irradiation, each experimental fish were observed for its morphological, behavioral, clinical symptoms, antioxidant levels and DNA damage were evaluated. Among the experimental groups 5ppm shilajit encapsulated Chironomous larvae fed fish group shows the most significant radioprotective effects compared with control and other experimental fish groups. The present study indicates that shilajit have significant radioprotective and antioxidant enhancing capability. The humus substance of shilajit may be the factor responsible to react with radiation-derived or radiation related reactive species on zebrafish.
以斑马鱼（Danio rerio）作为模式生物，我们评估了印度传统药物希拉季特在X射线照射下的辐射防护和抗氧化作用。斑马鱼被分为三个实验组和一个对照组，每组包含十条鱼。三个实验鱼组，即第一组、第二组和第三组，分别投喂含有3ppm、5ppm和7ppm希拉季特的包囊摇蚊幼虫，第四组作为对照组，投喂未包囊的幼虫。经过60天的喂养试验后，鱼接受单次急性剂量1Gy的X射线照射。照射后72小时，观察每条实验鱼的形态、行为、临床症状，评估其抗氧化水平和DNA损伤情况。在实验组中，与对照组和其他实验鱼组相比，投喂含有5ppm希拉季特的包囊摇蚊幼虫的鱼组显示出最显著的辐射防护作用。本研究表明，希拉季特具有显著的辐射防护和增强抗氧化能力。希拉季特的腐殖质物质可能是与斑马鱼上辐射衍生或与辐射相关的活性物质发生反应的因素。
