Fredi André, Nolis Pau, Parella Teodor
Servei de Ressonància Magnètica Nuclear, Facultat de Ciències, Universitat Autònoma de Barcelona, E-08193, Bellaterra (Barcelona), Catalonia, Spain.
Magn Reson Chem. 2017 Jun;55(6):525-529. doi: 10.1002/mrc.4572. Epub 2017 Feb 20.
Selective refocusing (GSERF or the recent PSYCHEDELIC) experiments were originally designed to determine all proton-proton coupling constants (J ) for a selected proton resonance. They work for isolated signals on which selective excitation can be successfully applied but, as it happens in other selective experiments, fail for overlapped signals. To circumvent this limitation, a doubly-selective TOCSY-GSERF scheme is presented for the measurement of J in protons resonating in crowded regions. This new experiment takes advantage of the editing features of an initial TOCSY transfer to uncover hidden resonances that become accessible to perform the subsequent frequency-selective refocusing. Copyright © 2016 John Wiley & Sons, Ltd.
选择性重聚焦（GSERF或最近的PSYCHEDELIC）实验最初旨在确定选定质子共振的所有质子-质子耦合常数（J）。它们适用于能够成功应用选择性激发的孤立信号，但与其他选择性实验一样，对于重叠信号会失效。为了克服这一限制，本文提出了一种双选择性TOCSY-GSERF方案，用于测量在拥挤区域共振的质子的J值。这个新实验利用了初始TOCSY转移的编辑特性来揭示隐藏的共振，这些共振在后续的频率选择性重聚焦中变得可用。版权所有© 2016约翰威立父子有限公司。
