腐殖酸钠对大蒜试验中辐射诱导的染色体损伤的修饰作用。
Modification of radiation induced chromosome damage in Allium test by sodium humate.
作者信息
Shkarupa V M, Klymenko S V, Talko V V
机构信息
National Pirogov Memorial Medical University, 56 Pyrohova str., Vinnytsya, 21018, Ukraine.
State Institution National Research Center for Radiation Medicine of the National Academy of Medical Sciences of Ukraine, Melnykov str., 53, Kyiv, 04050, Ukraine.
出版信息
Probl Radiac Med Radiobiol. 2016 Dec;21:159-171.
OBJECTIVE
Cytogenetic analysis of the effects of sodium humate in induced γ irradiation mutagenesis in Allium test.
MATERIALS AND METHODS
Analysis of the root meristem cells of Allium cepa L. seeds carried by ana telophase. Inves tigated the effect of sodium humate (100 mg/l) on cytogenetic effects γ irradiation (137Cs) at doses of 5, 10 and 20 Gy.
RESULTS
Antimutagenic effect of sodium humate was revealed, what allows to consider it as potential therapeutic modifier of radiation damage. Antimutagenic effect of sodium humate increased with increasing irradiation dose. Spotted differential activity of the drug with respect to various types of aberrations are most effectively reducing the frequency of radiation markers mutagenesis - chromosomal aberrations manifested in lower efficiency of long term survivors against potential changes of chromosomes compared with short lived.
CONCLUSIONS
Revealed different mechanisms for implementing antimutagenic properties of sodium humate in con dition of γ irradiation induced mutagenesis in Allium test.
目的
在大蒜试验中对腐植酸钠在诱导γ射线诱变中的作用进行细胞遗传学分析。
材料与方法
分析处于末期的洋葱种子根分生组织细胞。研究了腐植酸钠（100毫克/升）对剂量为5、10和20戈瑞的γ射线（¹³⁷Cs）细胞遗传学效应的影响。
结果
揭示了腐植酸钠的抗诱变作用，这使其可被视为辐射损伤的潜在治疗修饰剂。腐植酸钠的抗诱变作用随辐射剂量增加而增强。该药物对各种类型畸变的差异活性表现为最有效地降低辐射标记诱变频率——与短命细胞相比，长期存活细胞中染色体畸变的效率较低，这体现了染色体潜在变化时染色体畸变的情况。
结论
揭示了在大蒜试验中γ射线诱导诱变条件下腐植酸钠实现抗诱变特性的不同机制。
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