Ito Hiroaki, Sato Jun, Tsujino Yukio, Yamaguchi Noriko, Kimura Satoshi, Gohda Keigo, Murakami Katsuhiro, Onimaru Manabu, Ohmori Tohru, Ishikawa Fumihiro, Inoue Haruhiro
Hiroaki Ito, Manabu Onimaru, Haruhiro Inoue, Department of Surgery, Digestive Disease Center, Showa University Koto Toyosu Hospital, Tokyo 135-8577, Japan.
World J Gastroenterol. 2016 Dec 14;22(46):10232-10241. doi: 10.3748/wjg.v22.i46.10232.
To analyse the long-term prognostic impact of circulating tumour cells (CTCs) in gastric cancer patients who underwent surgery.
A 7.5-mL peripheral vein blood sample was obtained from each patient with treatment-negative gastric adenocarcinoma before surgery. OBP-401, a telomerase-specific, replication-selective, oncolytic adenoviral agent carrying the green fluorescent protein gene, was used to label CTCs. Correlations between the number of CTCs and clinical end points were evaluated.
The median follow-up period of the surviving patients with gastric cancer was 60 mo. The CTC number tended to increase concomitantly with disease progression. The overall survival of patients with more than five CTCs in 7.5-mL of peripheral blood was lower than that of patients with five or less CTCs, although the difference was not significant ( = 0.183). A significant difference in relapse-free survival was found between patients with more than five and those with five or less CTCs ( = 0.034).
A lower number of CTCs was correlated with higher relapse-free survival rates in patients. Detection of CTCs using OBP-401 may be useful for predicting prognosis in gastric cancer.
分析循环肿瘤细胞（CTCs）对接受手术的胃癌患者的长期预后影响。
在手术前，从每位治疗阴性的胃腺癌患者采集7.5毫升外周静脉血样本。使用携带绿色荧光蛋白基因的端粒酶特异性、复制选择性溶瘤腺病毒制剂OBP - 401标记CTCs。评估CTCs数量与临床终点之间的相关性。
存活的胃癌患者中位随访期为60个月。CTCs数量往往随着疾病进展而增加。外周血7.5毫升中CTCs超过5个的患者总生存期低于CTCs为5个或更少的患者，尽管差异不显著（P = 0.183）。CTCs超过5个的患者与CTCs为5个或更少的患者之间无复发生存期存在显著差异（P = 0.034）。
较低的CTCs数量与患者较高的无复发生存率相关。使用OBP - 401检测CTCs可能有助于预测胃癌预后。
