Shen Xianjuan, Wang Mei, Guo Yuehua, Ju Shaoqing
Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, China.
Medical Office, Nantong University, China.
Adv Clin Exp Med. 2016 Sep-Oct;25(5):837-844. doi: 10.17219/acem/29182.
Lymphoma is a malignant tumor of the immune system originating from lymph nodes and extralymphatic tissues. Its occurrence is believed to be associated with various immune cells due to the proliferation and differentiation of lymphocytes during the immune response. It has been found in many studies that B-cell activating factor (BAFF), as a member of the tumor necrosis factor (TNF) superfamily, could specifically activate B lymphocytes and promote their proliferation.
To explore correlations between non-Hodgkin lymphoma (NHL) and the expression of BAFF and its receptors in NHL patients.
The protein expression of BAFF and its receptors in serum and BAFF mRNA expression in peripheral blood mononuclear cells (PBMCs) of 47 NHL patients and 20 healthy subjects were detected by ELISA and RFQ-PCR and compared with LDH and β2M levels.
BAFF mRNA expression in the PBMCs of NHL patients was significantly higher than in healthy controls. The expression levels of serum BAFF and the three receptors (TACI, BCMA and BAFF-R) in NHL patients were significantly higher than in healthy controls, and were not significantly correlated with β2M and LDH levels.
The serum protein concentration of BAFF and the expression level of BAFF mRNA in PBMCs of NHL patients underwent abnormal changes, indicating that BAFF and its receptors may play some role in the pathogenesis of NHL.
淋巴瘤是一种起源于淋巴结和淋巴外组织的免疫系统恶性肿瘤。由于免疫反应过程中淋巴细胞的增殖和分化，其发生被认为与多种免疫细胞有关。许多研究发现，作为肿瘤坏死因子（TNF）超家族成员的B细胞活化因子（BAFF）可特异性激活B淋巴细胞并促进其增殖。
探讨非霍奇金淋巴瘤（NHL）与NHL患者中BAFF及其受体表达之间的相关性。
采用ELISA和实时荧光定量PCR（RFQ-PCR）检测47例NHL患者和20例健康对照者血清中BAFF及其受体蛋白表达以及外周血单个核细胞（PBMCs）中BAFF mRNA表达，并与乳酸脱氢酶（LDH）和β2微球蛋白（β2M）水平进行比较。
NHL患者PBMCs中BAFF mRNA表达显著高于健康对照者。NHL患者血清BAFF及三种受体（TACI、BCMA和BAFF-R）表达水平显著高于健康对照者，且与β2M和LDH水平无显著相关性。
NHL患者血清BAFF蛋白浓度及PBMCs中BAFF mRNA表达水平发生异常变化，表明BAFF及其受体可能在NHL发病机制中发挥一定作用。
