Institute of Nutritional and Food Sciences, Chair of Bioanalytics , University of Bonn , Endenicher Allee 11-13 , D-53115 Bonn , Germany.
ELFI Analytik GbR , Massenhausener Straße 18a , D-85375 Neufahrn , Germany.
J Agric Food Chem. 2018 Mar 14;66(10):2403-2409. doi: 10.1021/acs.jafc.6b04747. Epub 2017 Jan 12.
The aroma profile of porcine fat from tainted boars, female pigs, and castrated male pigs was investigated by application of comparative aroma extract dilution analysis (cAEDA) on a SAFE distillate of volatiles prepared from porcine back fat samples. The AEDA resulted in a total of 16 aroma active compounds for boar fat with flavor dilution (FD) factors ranging from 2 to 2048, whereas 12 aroma active compounds were found in fat of female pigs and 14 in fat of castrated male pigs, both with FD factors ranging from 2 to 32. Odor activity values (OAVs) of key components for each fat were identified: In boar fat androstenone, skatole, indole, and 2-aminoacetophenone showed highest OAVs, whereas 2,5-dimethylpyrazine, 2,4-decadienal, and δ-decalactone showed highest OAVs in fat of female pigs. Fat of castrated male pigs showed highest OAVs for skatole, indole, 1-octen-3-ol and methional. Finally, the off-flavor attributes of boar fat were successfully simulated by a recombinant of all odorants at their natural concentration level in deodorized sunflower oil.
采用比较香气萃取稀释分析(cAEDA)法,对猪背脂样品的 SAFE 馏出物进行挥发性分析,研究了来自公猪、母猪和去势公猪的猪脂的香气特征。AEDA 共鉴定出 16 种猪脂的香气活性化合物,其风味稀释(FD)因子范围为 2 至 2048;母猪脂中鉴定出 12 种,FD 因子范围为 2 至 32;去势公猪脂中鉴定出 14 种,FD 因子范围为 2 至 32。确定了每种脂肪的关键成分的气味活度值(OAV):公猪脂中,雄甾酮、粪臭素、吲哚和 2-氨基苯乙酮的 OAV 最高,而母猪脂中,2,5-二甲基吡嗪、2,4-癸二烯醛和δ-癸内酯的 OAV 最高。去势公猪脂中,粪臭素、吲哚、1-辛烯-3-醇和甲硫醛的 OAV 最高。最后,在脱臭葵花籽油中,以天然浓度水平将所有气味物质复配,成功模拟了公猪脂的异味属性。