Moriki A
Nihon Geka Hokan. 1989 Jan 1;58(1):107-18.
We studied whether lymphokine-activated killer (LAK) cells and cytotoxic T lymphocytes (CTL) were capable of being induced in vitro from peripheral blood lymphocytes of patients with brain tumor. The LAK cells were generated by culturing recombinant IL-2 with peripheral blood lymphocytes. The culture was continued for 72 hours; then cytotoxicity to Hela cell was examined by 4-hr 51Cr release assay. LAK cells were induced from lymphocytes of patients with brain tumor, but the cytotoxicity was rather less than that of healthy subjects, and it was accompanied by clinical deterioration. CTL was generated by co-culture of patient's peripheral blood lymphocytes and autologous brain tumor cells with addition of rIL-2. The cytotoxicity to autologous and allogenic brain tumor cells was examined by 16-hr51Cr release assay. The cytotoxicity to autologous tumor was approximately 30-40%, and there was cross reaction to allogenic tumor cells. The adoptive transfer of CTL to four patients was performed. One patient improved clinically, and on CT scan, growth of the tumor appeared to have been reduced.
我们研究了是否能够从脑肿瘤患者的外周血淋巴细胞中体外诱导出淋巴因子激活的杀伤细胞（LAK细胞）和细胞毒性T淋巴细胞（CTL）。通过将重组白细胞介素-2（IL-2）与外周血淋巴细胞共同培养来产生LAK细胞。培养持续72小时；然后通过4小时的51铬释放试验检测对海拉细胞的细胞毒性。LAK细胞是从脑肿瘤患者的淋巴细胞中诱导产生的，但细胞毒性比健康受试者的要小得多，并且伴有临床病情恶化。CTL是通过将患者的外周血淋巴细胞与自体脑肿瘤细胞在添加rIL-2的情况下共同培养而产生的。通过16小时的51铬释放试验检测对自体和异体脑肿瘤细胞的细胞毒性。对自体肿瘤的细胞毒性约为30% - 40%，并且对异体肿瘤细胞有交叉反应。对4名患者进行了CTL的过继转移。1名患者临床症状改善，CT扫描显示肿瘤生长似乎有所减缓。
