Noulsri Egarit, Udomwinijsilp Prapaporn, Lerdwana Surada, Chongkolwatana Viroje, Permpikul Parichart
Research Division, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Department of Transfusion Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Transfus Apher Sci. 2017 Apr;56(2):135-140. doi: 10.1016/j.transci.2016.10.006. Epub 2016 Oct 25.
There has been an increased interest in platelet-derived microparticles (PMPs) in transfusion medicine. Little is known about PMP status during the preparation of platelet concentrates for transfusion.
The aim of this study is to compare the PMP levels in platelet components prepared using the buffy coat (BC), platelet-rich plasma platelet concentrate (PRP-PC), and apheresis (AP) processes.
Platelet components were prepared using the PRP-PC and BC processes. Apheresis platelets were prepared using the Trima Accel and Amicus instruments. The samples were incubated with annexin A5-FITC, CD41-PE, and CD62P-APC. At day 1 after processing, the PMPs and activated platelets were determined using flow cytometry.
Both the percentage and number of PMPs were higher in platelet components prepared using the Amicus instrument (2.6±1.8, 32802±19036 particles/μL) than in platelet components prepared using the Trima Accel instrument (0.5±0.4, 7568±5298 particles/μL), BC (1.2±0.6, 12,920±6426 particles/μL), and PRP-PC (0.9±0.6, 10731±5514 particles/μL). Both the percentage and number of activated platelets were higher in platelet components prepared using the Amicus instrument (33.2±13.9, 427553±196965 cells/μL) than in platelet components prepared using the Trima Accel instrument (16.2±6.1, 211209±87706 cells/μL), BC (12.9±3.2, 140624±41003 cells/μL), and PRP-PC (21.1±6.3, 265210±86257 cells/μL).
The study suggests high variability of PMPs and activated platelets in platelet components prepared using different processes. This result may be important in validating the instruments involved in platelet blood collection and processing.
输血医学中对血小板衍生微粒（PMPs）的关注日益增加。关于用于输血的血小板浓缩物制备过程中的PMPs状态知之甚少。
本研究旨在比较使用白膜层（BC）、富血小板血浆血小板浓缩物（PRP-PC）和单采术（AP）制备的血小板成分中的PMP水平。
使用PRP-PC和BC方法制备血小板成分。使用Trima Accel和Amicus仪器制备单采血小板。将样品与膜联蛋白A5-异硫氰酸荧光素（annexin A5-FITC）、CD41-藻红蛋白（CD41-PE）和CD62P-别藻青蛋白（CD62P-APC）一起孵育。在处理后第1天，使用流式细胞术测定PMPs和活化血小板。
使用Amicus仪器制备的血小板成分中PMPs的百分比和数量（2.6±1.8，32802±19036个颗粒/微升）高于使用Trima Accel仪器（0.5±0.4，7568±5298个颗粒/微升）、BC（1.2±0.6，12920±6426个颗粒/微升）和PRP-PC（0.9±0.6，10731±5514个颗粒/微升）制备的血小板成分。使用Amicus仪器制备的血小板成分中活化血小板的百分比和数量（33.2±13.9，427553±196965个细胞/微升）高于使用Trima Accel仪器（16.2±6.1，211209±87706个细胞/微升）、BC（12.9±3.2，140624±41003个细胞/微升）和PRP-PC（21.1±6.3，265210±86257个细胞/微升）制备的血小板成分。
该研究表明，使用不同方法制备的血小板成分中PMPs和活化血小板存在高度变异性。这一结果对于验证参与血小板采集和处理的仪器可能具有重要意义。
