Ahrazem Oussama, Argandoña Javier, Castillo Raquel, Rubio-Moraga Ángela, Gómez-Gómez Lourdes
Instituto Botánico, Departamento de Ciencia y Tecnología Agroforestal y Genética, Facultad de Farmacia, Universidad de Castilla-La Mancha, Albacete, Spain.
Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Toledo, Spain.
PLoS One. 2016 Dec 28;11(12):e0168736. doi: 10.1371/journal.pone.0168736. eCollection 2016.
Using a subtractive hybridization approach, differentially expressed genes involved in the light response in saffron stigmas were identified. Twenty-two differentially expressed transcript-derived fragments were cloned and sequenced. Two of them were highly induced by light and had sequence similarity to early inducible proteins (ELIP) and SOUL heme-binding proteins. Using these sequences, we searched for other family members expressed in saffron stigma. ELIP and SOUL are represented by small gene families in saffron, with four and five members, respectively. The expression of these genes was analyzed during the development of the stigma and in light and dark conditions. ELIP transcripts were detected in all the developmental stages showing much higher expression levels in the developed stigmas of saffron and all were up-regulated by light but at different levels. By contrast, only one SOUL gene was up-regulated by light and was highly expressed in the stigma at anthesis. Both the ELIP and SOUL genes induced by light in saffron stigmas might be associated with the structural changes affecting the chromoplast of the stigma, as a result of light exposure, which promotes the development and increases the number of plastoglobules, specialized in the recruitment of specific proteins, which enables them to act in metabolite synthesis and disposal under changing environmental conditions and developmental stages.
采用消减杂交方法，鉴定了藏红花柱头中参与光反应的差异表达基因。克隆并测序了22个差异表达的转录本衍生片段。其中两个受光高度诱导，与早期诱导蛋白（ELIP）和SOUL血红素结合蛋白具有序列相似性。利用这些序列，我们搜索了藏红花柱头中表达的其他家族成员。ELIP和SOUL在藏红花中分别由小基因家族代表，分别有四个和五个成员。在柱头发育过程中以及光照和黑暗条件下分析了这些基因的表达。在所有发育阶段均检测到ELIP转录本，在藏红花发育成熟的柱头中表达水平高得多，并且均受光上调，但上调水平不同。相比之下，只有一个SOUL基因受光上调，并且在开花期的柱头中高表达。藏红花柱头中受光诱导的ELIP和SOUL基因可能都与光照导致的柱头有色体结构变化有关，光照促进了质体小球的发育并增加了其数量，质体小球专门招募特定蛋白质，使其能够在不断变化的环境条件和发育阶段中参与代谢物的合成和处理。
