Martinek P, Kula E, Hedbávný J
Department of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemedelská 3, 613 00 Brno, Czech Republic (
Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelská 1, 613 00 Brno, Czech Republic (
Environ Entomol. 2017 Feb 1;46(1):131-136. doi: 10.1093/ee/nvw144.
Reaction of leaf weevil (Phyllobius arborator (Herbst)) to increased concentration of manganese in diet was investigated in laboratory rearing with controlled temperature, humidity, and light conditions. Food for leaf weevils in rearing (leaves of birch Betula pendula Roth) was contaminated by soaking the leaves in solutions of MnCl2.4H2O with graded concentration of manganese. Direct influence of food was characterized by the consumed amount of leaves, period of feeding, and weight of P. arborator adults. At the same time, the levels of manganese in unconsumed food, excrement, and bodies of adults were determined.Even very high content of manganese in food did not cause significantly different reaction of P. arborator adults in comparison to individuals in control treatment. No significant difference in the quantity of the consumed food, weight of adults, and duration of their feeding period was found between the treatments within the experiment. The content of manganese found in food, excrement, and adult beetles indicate that P. arborator avoided manganese intoxication through food by both-voiding manganese through the feces and sequestering it at relatively high concentrations in unspecified parts of their body.
在温度、湿度和光照条件可控的实验室饲养环境中，研究了叶象甲（Phyllobius arborator (Herbst)）对日粮中锰浓度升高的反应。饲养叶象甲的食物（垂枝桦Betula pendula Roth的叶子）通过将叶子浸泡在不同锰浓度的MnCl2·4H2O溶液中进行污染。食物的直接影响通过叶子的消耗量、取食时间和叶象甲成虫的体重来表征。同时，测定了未消耗食物、粪便和成虫体内的锰含量。与对照处理的个体相比，即使食物中锰含量非常高，叶象甲成虫的反应也没有显著差异。在实验中的各处理之间，未发现食物消耗量、成虫体重及其取食期持续时间有显著差异。在食物、粪便和成虫甲虫中发现的锰含量表明，叶象甲通过粪便排出锰以及将锰以相对高浓度螯合在其身体未明确部位，从而避免通过食物发生锰中毒。
