Yang Shuai, Mei Xiang-Dong, Zhang Xiao-Fang, Li Yao-Fa, She Dongmei, Zhang Tao, Ning Jun
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No 2 West Yuan Ming Yuan Road, Beijing, 100193, China.
Institute of Plant Protection, Integrated Pest Management Center of Hebei Province, Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture, Hebei Academy of Agricultural and Forestry Sciences, 437 Dongguan Street, Baoding City, Hebei Province, 071000, China.
J Chem Ecol. 2017 Feb;43(2):180-187. doi: 10.1007/s10886-016-0809-5. Epub 2016 Dec 29.
The coffee bean weevil (CBW), Araecerus fasciculatus (De Geer, 1775) (Coleoptera: Anthribidae) is an important pest of stored products such as grains, coffee beans, cassava, and traditional Chinese medicine materials. In China, CBW causes large losses of Daqu, a traditional Chinese liquor fermentation starter, and, unfortunately, the use of conventional insecticides against CBW is not suitable in Daqu storage. We found CBW to be highly attracted to fermenting yeast cultures, such as Kluyveromyces lactis. Eight volatile compounds, produced by fermenting cultures and not by sterile samples, were identified by gas chromatography coupled with mass spectrometry. Five of these substances elicited significant responses in Y-tube behavioral bioassays. Field trapping experiments revealed 2-phenylethanol and 2-phenylethyl acetate to be crucial for attraction of CBW. Results show that yeast volatiles play an important role in host location, and that 2-phenylethanol and 2-phenylethyl acetate could be utilized as potential attractants in monitoring and control systems against this important pest.
咖啡豆象（CBW），即咖啡豆象（Araecerus fasciculatus (De Geer, 1775)）（鞘翅目：长蠹科），是谷物、咖啡豆、木薯和中药材等储藏产品的重要害虫。在中国，咖啡豆象会给大曲（一种传统白酒发酵酒曲）造成巨大损失，不幸的是，使用常规杀虫剂防治咖啡豆象并不适用于大曲储存。我们发现咖啡豆象对发酵酵母培养物（如乳酸克鲁维酵母）具有高度吸引力。通过气相色谱-质谱联用技术鉴定出八种由发酵培养物产生而非无菌样品产生的挥发性化合物。其中五种物质在Y型管行为生物测定中引发了显著反应。田间诱捕实验表明，2-苯乙醇和乙酸苯乙酯对吸引咖啡豆象至关重要。结果表明，酵母挥发物在寄主定位中起重要作用，并且2-苯乙醇和乙酸苯乙酯可作为潜在引诱剂用于针对这种重要害虫的监测和控制系统。
