Bioseparation Engineering Group, Department of Mechanical Engineering, Technical University of Munich, München, Germany.
J Sep Sci. 2017 Mar;40(5):1176-1183. doi: 10.1002/jssc.201601102. Epub 2017 Jan 27.
Electrochemically modulated liquid chromatography is a special form of ion exchange chromatography in which the separation process is controlled by applying an electric potential to the stationary phase. This form of chromatography has so far only been applied in research studies. The present study shows that multiwalled carbon nanotubes are an effective resin material for an electrochemically modulated chromatography process. The experiments are carried out in a newly designed column that enables the packing of nanomaterials. We investigate the influence of the applied potential on the retention and elution of maleic acid, determine the dynamic binding capacity, and calculate the utilization degree of the electrical charge in the adsorption process. Moreover, the stability of the resin and the membrane over more than 200 working hours are presented. In addition to the stability, their sturdiness and inexpensive price are important qualities that make multiwalled carbon nanotubes interesting for application as the stationary phase in an electrochemically driven process. The investigated chromatography technique represents a promising separation process for future applications as a preparative step in biotechnology as well as other life science fields.
电化学调制液相色谱是一种特殊的离子交换色谱，其中分离过程通过对固定相施加电势来控制。这种形式的色谱法迄今仅应用于研究中。本研究表明，多壁碳纳米管是电化学调制色谱过程中的一种有效树脂材料。实验在新设计的柱中进行，该柱可实现纳米材料的填充。我们研究了施加电势对马来酸保留和洗脱的影响，确定了动态结合容量，并计算了吸附过程中电荷的利用率。此外，还介绍了树脂和膜超过 200 个工作小时的稳定性。除了稳定性之外，多壁碳纳米管作为电化学驱动过程中固定相的应用具有坚固耐用且价格低廉等重要特性，这使其具有吸引力。所研究的色谱技术代表了一种有前途的分离过程，可作为生物技术以及其他生命科学领域的制备步骤的未来应用。
