Xu L, Huang H-J, Zhou X, Liu C-W, Bao Y-Y
State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences Zhejiang University, Hangzhou, 310058, China.
Insect Mol Biol. 2017 Jun;26(3):277-285. doi: 10.1111/imb.12290. Epub 2016 Dec 29.
In this study, we identified a pancreatic lipase-related protein 2 (PLRP2) gene through searching the transcriptome data of the brown planthopper, Nilaparvata lugens, a monophagous rice pest. PLRP2 mRNAs were first isolated in the human pancreas and play an important role in hydrolysis of galactolipids from the diet. Although homologous PLRP2 genes have been identified in many insect species, their physiological functions remain unknown. The present study for the first time reports the functional role of PLRP2 in an insect species. Differing from mammal PLRP2s, N. lugens PLRP2 was highly expressed in developing oocytes of the ovaries of female adults and little expressed in laid eggs. Suppression of N. lugens PLRP2 expression using RNA interference significantly inhibited egg hatching in rice seedlings, implying that N. lugens PLRP2 was important for oocyte maturation and development. This novel finding will improve our understanding of the reproductive strategies in insects and provides a potential target for future management of crop pests.
在本研究中，我们通过搜索褐飞虱（一种单食性水稻害虫）的转录组数据，鉴定出了一种胰腺脂肪酶相关蛋白2（PLRP2）基因。PLRP2 mRNA最初是在人类胰腺中分离出来的，在饮食中半乳糖脂的水解过程中发挥重要作用。尽管在许多昆虫物种中都已鉴定出同源的PLRP2基因，但其生理功能仍然未知。本研究首次报道了PLRP2在一种昆虫物种中的功能作用。与哺乳动物的PLRP2不同，褐飞虱的PLRP2在雌性成虫卵巢发育中的卵母细胞中高度表达，而在产下的卵中表达很少。使用RNA干扰抑制褐飞虱PLRP2的表达显著抑制了水稻幼苗中的卵孵化，这意味着褐飞虱PLRP2对卵母细胞的成熟和发育很重要。这一新发现将增进我们对昆虫繁殖策略的理解，并为未来农作物害虫的治理提供一个潜在靶点。
