Wratten M L, Gratton E, van de Ven M, Sevanian A
Department of Pathology and Toxicology, University of Southern California, Los Angeles 90033.
Biochem Biophys Res Commun. 1989 Oct 16;164(1):169-75. doi: 10.1016/0006-291x(89)91698-7.
Multifrequency phase fluorometry was used to determine the lifetime distributions of 1,6 diphenyl-1,3,5-hexatriene in 1-palmitoyl-2 linoleoyl phosphatidylcholine small unilamellar vesicles containing 2% incorporation of phospholipid hydroperoxides. A biexponential decay was observed in both vesicle preparations over a temperature range of 5 to 35 degrees C. Vesicles containing phospholipid hydroperoxides showed an overall longer lifetime as well as a greater distribution in width. These findings suggest that phospholipid hydroperoxides create structural heterogeneity in membrane structure.
多频相荧光法用于测定1,6 - 二苯基 - 1,3,5 - 己三烯在含有2%磷脂氢过氧化物掺入量的1 - 棕榈酰 - 2 - 亚油酰磷脂酰胆碱小单层囊泡中的寿命分布。在5至35摄氏度的温度范围内，两种囊泡制剂均观察到双指数衰减。含有磷脂氢过氧化物的囊泡显示出总体更长的寿命以及更大的宽度分布。这些发现表明磷脂氢过氧化物在膜结构中产生了结构异质性。
