Songsivilai S, Clissold P M, Lachmann P J
MRC Molecular Immunopathology Unit, Medical Research Council Centre, Cambridge, England.
Biochem Biophys Res Commun. 1989 Oct 16;164(1):271-6. doi: 10.1016/0006-291x(89)91713-0.
We have used genetic manipulation to produce chimeric bispecific antibodies. Plasmids containing variable regions of immunoglobulin from a murine hybridoma secreting anti-hepatitis B surface antigen were joined to human constant regions. These chimeric plasmids were introduced into transfectomas, secreting chimeric antibodies to iodo-hydroxy-nitrophenyl, by electroporation. Transfectomas secreting bispecific antibodies were identified. This approach has advantages over the fusion of hybridomas or chemical linking of two antibody molecules and will enable the use of bispecific antibodies in vivo.
