Zheng Qi
Department of Epidemiology and Biostatistics, Texas A&M School of Public Health, College Station, TX 77843, United States.
Mutat Res. 2017 Jan;795:10-14. doi: 10.1016/j.mrfmmm.2016.12.001. Epub 2016 Dec 19.
The Luria-Delbrück fluctuation experiment protocol is increasingly employed to determine microbial mutation rates in the laboratory. An important question raised at the planning stage is "How many cultures are needed?" For over 70 years sample sizes have been determined either by intuition or by following published examples where sample sizes were chosen intuitively. This paper proposes a practical method for determining the sample size. The proposed method relies on existing algorithms for computing the expected Fisher information under two commonly used mutant distributions. The role of partial plating in reducing sample size is discussed.
鲁里亚-德尔布吕克波动实验方案在实验室中越来越多地用于确定微生物的突变率。在规划阶段提出的一个重要问题是“需要多少培养物？”七十多年来，样本量要么凭直觉确定，要么遵循已发表的凭直觉选择样本量的示例。本文提出了一种确定样本量的实用方法。所提出的方法依赖于现有的算法，用于在两种常用的突变分布下计算期望费希尔信息。讨论了稀释平板法在减小样本量方面的作用。
