Zhang L, Castellino F J
Department of Chemistry, University of Notre Dame, Indiana 46556.
J Protein Chem. 1989 Aug;8(4):471-80. doi: 10.1007/BF01026431.
Rabbit polyclonal antibodies to a synthetic peptide, NH2-Asp-Thr-Asn-Gln-Val-Asp-Gln-Lys-Asp-Gln-Leu-Asp-Phe-Arg-CONH2 (A Pep), have been produced. This sequence is identical to that contained in the tetradecapeptide released from bovine protein C (PC) as a result of its conversion to its activated form (APC), except that Phe13 replaced the normal Pro13, in order to discourage cross-reactivity of antibodies to the carboxylterminal portion of APep with PC. The antibody pool obtained reacted with PC and showed virtually no cross-reactivity toward either APC or several typical plasma proteins. This general approach should serve well as a means of production of antibodies with a designed specificity capable of distinguishing between forms of the same protein that arise by release of peptide material.
已制备出针对合成肽NH2-Asp-Thr-Asn-Gln-Val-Asp-Gln-Lys-Asp-Gln-Leu-Asp-Phe-Arg-CONH2（A Pep）的兔多克隆抗体。该序列与牛蛋白C（PC）转化为活化形式（APC）后释放的十四肽中的序列相同，只是Phe13取代了正常的Pro13，以防止抗体与APep羧基末端部分与PC发生交叉反应。所获得的抗体池与PC反应，对APC或几种典型血浆蛋白几乎没有交叉反应。这种通用方法作为一种生产具有特定设计特异性的抗体的手段，能够区分通过肽物质释放产生的同一蛋白质的不同形式，应该会很有用。
