Tang Y Q, Tian S H, Wu S X, Hua J C, Wu G F, Zhao E M, Lu Y A, Zhu Y Q, Zou G, Tsou K
Shanghai Institute of Materia Medica, Academia Sinica.
Sci China B. 1989 May;32(5):570-9.
A new tetradecapeptide, ranamargarin, has been isolated by Sep-Pak C18 and HPLC from methanol extracts of the skin of the Chinese frog Rana margaratae. The sequence of the peptide is: Asp-Asp-Ala-Ser-Asp-Arg-Ala-Lys-Lys-Phe-Tyr-Gly-Leu-Met-NH2. This structure has been confirmed by synthesis. The peptide is the largest among the amphibian tachykinins and its N-terminal amino acids are quite different from those of the other tachykinins. The formation of the sulfoxide and peak-splitting of ranamargarin during purification procedures are briefly discussed.
一种新的十四肽——林蛙皮素，已通过Sep - Pak C18和高效液相色谱法从中国林蛙皮肤的甲醇提取物中分离出来。该肽的序列为：天冬氨酸 - 天冬氨酸 - 丙氨酸 - 丝氨酸 - 天冬氨酸 - 精氨酸 - 丙氨酸 - 赖氨酸 - 赖氨酸 - 苯丙氨酸 - 酪氨酸 - 甘氨酸 - 亮氨酸 - 甲硫氨酸 - 氨基。此结构已通过合成得到证实。该肽是两栖类速激肽中最大的，其N端氨基酸与其他速激肽的N端氨基酸有很大不同。文中简要讨论了林蛙皮素在纯化过程中形成亚砜及峰分裂的情况。
